Human beta -Catenin Alexa Fluor® 647-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human, mouse and rat beta -Catenin in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human beta -Catenin
    Accession # P35222
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human beta ‑Catenin Alexa Fluor® 647‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1329R, filled histogram) or isotype control antibody (Catalog # IC108R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-Catenin
beta -Catenin is a 94-96 kDa member of the beta -Catenin family, armadillo-repeat superfamily of intracellular molecules. Human beta -Catenin is 781 amino acids (aa) in length and contains three basic regions; there is a "flexible" N-terminus (aa 1-110) that undergoes phosphorylation, O-linked glycosylation, acetylation and ubiquitination, a central region (aa 14-670) that contains 12 armadillo-like repeats and engages in protein-protein interaction, and a "flexible" C-terminus that also undergoes phosphorylation. beta -Catenin is widely, if not ubiquitously expressed, being found in endothelial cells, NKT cells, cortical neurons, neural stem cells, osteoblasts, fibroblasts, skeletal muscle cells, and Tregs. beta -Catenin is perhaps best known as a facilitator for Wnt signaling. In this context, and in the absence of Wnt signaling, non-nuclear or E-Cadherin-associated beta -Catenin is continually synthesized and degraded via a phosphorylation-dependent program. Upon Wnt engagement of its LPR:Fzd receptor complex, phosphorylation stops, allowing for an increase in it half-life and translocation into the nucleus. Here, it binds to TCF, generating a gene-activating transcriptional complex. Notably, Wnt signaling has been described as being  beta -Catenin and Ca++/non-beta -Catenin based. It now appears they work in tandem, with Ca++ mobilization facilitating the translocation of beta -Catenin across the nuclear membrane. Human and mouse full-length beta -Catenin shows 99% aa sequence identity.
  • Entrez Gene IDs:
    1499 (Human); 12387 (Mouse); 84353 (Rat)
  • Alternate Names:
    bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG Alexa Fluor® 647-conjugated Antibody

Ctrl IC108R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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