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Human beta-Synuclein Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5528U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5528U-100UG

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Human beta-Synuclein Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -Synuclein in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) alpha -Synuclein and less than 1% cross-reactivity with rh gamma -Synuclein is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human β-Synuclein
Gly47-Glu120
Accession # Q16143
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: beta-Synuclein

beta -Synuclein is a 19 kDa, 134 amino acid (aa) cytoplasmic phosphoprotein that is a member of a family of small, highly conserved proteins, including synucleins alpha, beta, and gamma (synoretin), localized predominantly in presynaptic nerve terminals in the brain. beta -Synuclein  associates with alpha -Synuclein, preventing it from aggregating. Abnormal alpha -Synuclein aggregates form filamentous inclusions (Lewy bodies) in neurodegenerative diseases including Parkinson's disease. Within the region used as an immunogen, human SNCB shares 99% aa identity with mouse and rat beta -Synuclein.

Entrez Gene IDs
6620 (Human); 104069 (Mouse); 113893 (Rat)
Alternate Names
beta-Synuclein; SNCB; synuclein, beta; Synuclein-beta

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