Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1‑4). The human beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (1). Human beta IG-H3 shares 91% and 93% aa sequence identity with mouse and porcine beta IG-H3, respectively. beta IG-H3 is expressed as a 75 kDa protein with no post‑translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5,6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 binding to matrix Fibronectin, Collagen I, and Collagen VI (3, 8‑10) in addition to cell expressed Integrins alpha V beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 11, 12). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 11, 13). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8,11). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (12, 13). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (14). beta IG-H3 is downregulated in many cancers (4, 15) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 15).
Human beta IG‑H3 Biotinylated Antibody
R&D Systems | Catalog # BAF2935
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta IG-H3
Gly24-His683
Accession # Q15582
Gly24-His683
Accession # Q15582
Specificity
Detects human beta IG-H3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse beta IG-H3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human beta IG‑H3 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human beta IG-H3 (Catalog # 3409-BG)
Sample: Recombinant Human beta IG-H3 (Catalog # 3409-BG)
Human beta IG-H3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using BAF2935 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: beta IG-H3
References
- Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
- Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
- Lee, S-H. et al. (2003) Kidney Int. 64:1012.
- Zhao, Y.L. et al. (2002) Oncogene 21:7471.
- Andersen, R.B. et al. (2004) Biochemistry 43:16374.
- Kim, J-E. et al. (2003) Oncogene 22:2045.
- Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
- Thapa, N. et al. (2005) Bone 36:232.
- Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
- Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
- Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
- Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
- Cao, W, et al. (2006) Blood 107:2777.
- Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
- Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.
Long Name
TGF-beta Induced Gene H3
Alternate Names
beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP
Gene Symbol
TGFBI
UniProt
Additional beta IG-H3 Products
Product Documents for Human beta IG‑H3 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human beta IG‑H3 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Human beta IG‑H3 Biotinylated Antibody
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Customer Reviews for Human beta IG‑H3 Biotinylated Antibody (2)
4 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: See PMID 22769442Species: HumanVerified Customer | Posted 01/12/2015
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Application: ImmunocytochemistrySample Tested: See PMID 22769442Species: HumanVerified Customer | Posted 01/12/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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