Human beta IG-H3 Antibody [Biotin]

R&D Systems | Catalog # BAF2935

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all beta IG-H3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta IG-H3
Gly24-His683
Accession # Q15582

Specificity

Detects human beta IG-H3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse beta IG-H3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human beta IG-H3 Antibody [Biotin]

Human beta IG-H3 ELISA Standard Curve

Human beta IG-H3 ELISA Standard Curve

Recombinant Human beta IG‑H3 (Catalog # 3409-BG) was serially diluted and captured by Mouse Anti-Human beta IG‑H3 Monoclonal Antibody (Catalog # MAB2935) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human beta IG‑H3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2935) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human beta IG-H3 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human beta IG-H3 (Catalog # 3409-BG)

Human beta IG-H3 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human beta IG‑H3 Antibody (Catalog # MAB2935)
  • Standard: Recombinant Human beta IG-H3 Protein, CF (Catalog # 3409-BG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using BAF2935 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta IG-H3

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1‑4). The human beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (1). Human beta IG-H3 shares 91% and 93% aa sequence identity with mouse and porcine beta IG-H3, respectively. beta IG-H3 is expressed as a 75 kDa protein with no post‑translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5,6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 binding to matrix Fibronectin, Collagen I, and Collagen VI (3, 8‑10) in addition to cell expressed Integrins alpha V beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 11, 12). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 11, 13). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8,11). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (12, 13). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (14). beta IG-H3 is downregulated in many cancers (4, 15) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 15).

References

  1. Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
  2. Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
  3. Lee, S-H. et al. (2003) Kidney Int. 64:1012.
  4. Zhao, Y.L. et al. (2002) Oncogene 21:7471.
  5. Andersen, R.B. et al. (2004) Biochemistry 43:16374.
  6. Kim, J-E. et al. (2003) Oncogene 22:2045.
  7. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
  8. Thapa, N. et al. (2005) Bone 36:232.
  9. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
  10. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
  11. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
  12. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
  13. Cao, W, et al. (2006) Blood 107:2777. 
  14. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
  15. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.

Long Name

TGF-beta Induced Gene H3

Alternate Names

beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP

Entrez Gene IDs

7045 (Human); 21810 (Mouse)

Gene Symbol

TGFBI

UniProt

Q15582

Additional beta IG-H3 Products

Product Documents for Human beta IG-H3 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human beta IG-H3 Antibody [Biotin]

For research use only

Citations for Human beta IG-H3 Antibody [Biotin]

Customer Reviews for Human beta IG-H3 Antibody [Biotin] (2)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22769442
    Species: Human
    Verified Customer | Posted 01/12/2015
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 22769442
    Species: Human
    Verified Customer | Posted 01/12/2015

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