Human BMPR-IB/ALK-6 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5051P
Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry.
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Citations (3)
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Human BMPR-IB/ALK-6 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMPR‑IB/ALK‑6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse BMPR-IB is observed.
Source
Monoclonal Mouse IgG2B Clone # 477914
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR‑IB/ALK‑6
Lys14-Arg126
Accession # O00238
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry. View Larger

Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR‑IB/ALK‑6 PE‑conjugated Monoclonal Antibody (Catalog # FAB5051P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of BMPR-IB/ALK-6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry. View Larger

Detection of BMPR-IB/ALK-6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry. Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 PE-conjugated Monoclonal Antibody (Catalog # FAB5051P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

References
  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
Long Name
Bone Morphogenetic Protein Receptor IB
Entrez Gene IDs
658 (Human); 12167 (Mouse)
Alternate Names
activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase

Product Datasheets

Citations for Human BMPR-IB/ALK-6 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps
    Authors: L Tengroth, J Arebro, O Larsson, C Bachert, SK Georén, LO Cardell
    Sci Rep, 2018;8(1):1561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. A Selective Cell Population From Dermis Strengthens Bone Regeneration
    Stem Cells Transl Med, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell selection
  3. Bone morphogenetic protein receptor IB as a marker for enrichment of osteogenic precursor-like cells in human dermis.
    Authors: He J, Dong J, Wang T
    Arch. Dermatol. Res., 2011;303(8):581-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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