Human BMPR-IB/ALK-6 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human BMPR‑IB/ALK‑6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse BMPR-IB is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 477914
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BMPR‑IB/ALK‑6
    Lys14-Arg126
    Accession # O00238
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR‑IB/
ALK‑6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Immunocytochemistry
BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line. BMPR‑IB/ALK‑6 was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human
BMPR‑IB/ALK‑6 Monoclonal Antibody (Catalog # MAB5051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

  • References:
    1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
    2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
  • Long Name:
    Bone Morphogenetic Protein Receptor IB
  • Entrez Gene IDs:
    658 (Human); 12167 (Mouse)
  • Alternate Names:
    activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human BMPR-IB/ALK-6 Antibody MAB5051
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 Flow Human Anonymous 10/20/2017
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Flow Cytometry Human BMPR-IB/ALK-6 Antibody MAB5051
Flow Cytometry: Human BMPR-IB/ALK-6 Antibody [MAB5051]

Summary

ApplicationFlow Cytometry
Sample TestedCells from neural differentiation of hiPSCs
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe cells were stained with 1µl of anti-BMPR1 antibody in 100 µl FACS staining buffer for 30 min at 4C. After washing, the cells were incubated with 1µl of anti-mouse IgG2b-APC antibody in 100 µl FACS staining buffer for 20 min at 4C.

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