Human BRIP1/FANCJ Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6496U Datasheet / COA / SDS

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FAB6496U-100UG

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All BRIP1/FANCJ Antibodies

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Human BRIP1/FANCJ Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BRIP1/FANCJ in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FANCA, D2, E, F, G, I, L, M, or recombinant mouse BRIP1/FANCJ is observed.

Source

Monoclonal Mouse IgG1 Clone # 652747

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human BRIP1/FANCJ
Ser2-Arg160
Accession # Q9BX63

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BRIP1/FANCJ

BRCA1-interacting protein 1 (BRIP1), also known as Fanconi anemia complementation group J protein (FANCJ) and BACH1, is a 130 kDa ubiquitously expressed DNA helicase. BRIP1 binds to the BRCT domain of BRCA1 and regulates BRCA1 dependent DNA repair and cell cycle checkpoint control. It is mutated in patients with Fanconi anemia, a disorder characterized by chromosomal instability and increased risk of developing cancer. Within amino acids 1-160, human BRIP1 shares 78% and 74% aa sequence identity with mouse and rat BRIP1, respectively.

Long Name

BRCA1 Interacting Protein C-terminal Helicase 1

Entrez Gene IDs

83990 (Human)

Alternate Names

ATP-dependent RNA helicase BRIP1; BACH1; BACH1BRCA1/BRCA2-associated helicase 1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRIP1; EC 3.6.1; EC 3.6.4.13; FACJ; FANCJ; FANCJFanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ