Human Cadherin‑12 Antibody

R&D Systems | Catalog # MAB2240

R&D Systems
Discontinued Product
MAB2240 has been discontinued. View all Cadherin-12 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Surface Plasmon Resonance (SPR)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 343621
View all Cadherin-12 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin-12
Met1-Pro609
Accession # P55289

Specificity

Detects human Cadherin‑12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -13, -17, rhE-Cadherin, rhP-Cadherin, rhR-Cadherin, or rhVR-Cadherin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human Cadherin‑12 Antibody

Cadherin-12 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Cadherin‑12 in BG01V Human Embryonic Stem Cells.

Cadherin-12 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Rat Anti-Human Cadherin-12 Monoclonal Antibody (Catalog # MAB2240) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Cadherin‑12 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample:

Human neural progenitor cells differentiated by growth factor withdrawal

 

Note: Since classic Cadherins can be protected from trypsin treatment in the presence of Ca2+, cells in monolayer cultures are harvested with 0.01% Trypsin in the presence of 1-5 mM CaCl2 at 37° C. Flow cytometry can be performed according to the standard procedures, except that all the cell staining and washing steps are performed in the presence of Ca2+ and Mg2+ (e.g. using FACS buffer: PBS containing 1 mM CaCl2, 1 mM MgCl2, 2% FBS and 0.02% sodium azide).

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal and BG01V human embryonic stem cells differentiated into neurons

Western Blot

1 µg/mL
Sample: Recombinant Human Pro Cadherin‑12 Fc Chimera (Catalog # 2240-CA)

Reviewed Applications

Read 1 review rated 5 using MAB2240 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cadherin-12

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1).
Cadherin‑12, also known as brain-cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium-dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin-12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).

References

  1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  4. Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398.
  5. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  6. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.

Alternate Names

Br-Cadherin, Cadherin12, CDH12, CDHB, N-Cadherin 2

Entrez Gene IDs

1010 (Human)

Gene Symbol

CDH12

UniProt

P55289

Additional Cadherin-12 Products

Product Documents for Human Cadherin‑12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cadherin‑12 Antibody

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Human Cadherin‑12 Antibody

Customer Reviews for Human Cadherin‑12 Antibody (1)

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  • Human Cadherin-12 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Glioma cells
    Species: Human
    Verified Customer | Posted 10/07/2022
    Human Cadherin‑12 Antibody MAB2240

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Protocols

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FAQs for Human Cadherin‑12 Antibody

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    • Q: Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?
      A: The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 
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