Human Cadherin-12 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Cadherin‑12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -13, -17, rhE-Cadherin, rhP-Cadherin, rhR-Cadherin, or rhVR-Cadherin is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 343621
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin-12
    Met1-Pro609
    Accession # P55289
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Pro Cadherin‑12 Fc Chimera (Catalog # 2240-CA)
  • Flow Cytometry
    0.25 µg/106 cells

    Human neural progenitor cells differentiated by growth factor withdrawal

     

    Note: Since classic Cadherins can be protected from trypsin treatment in the presence of Ca2+, cells in monolayer cultures are harvested with 0.01% Trypsin in the presence of 1-5 mM CaCl2 at 37° C. Flow cytometry can be performed according to the standard procedures, except that all the cell staining and washing steps are performed in the presence of Ca2+ and Mg2+ (e.g. using FACS buffer: PBS containing 1 mM CaCl2, 1 mM MgCl2, 2% FBS and 0.02% sodium azide).

  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Cadherin‑12 in BG01V Human Embryonic Stem Cells. Cadherin‑12 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Rat Anti-Human Cadherin‑12 Monoclonal Antibody (Catalog # MAB2240) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cadherin-12

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1).
Cadherin‑12, also known as brain-cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium-dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin-12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).

  • References:
    1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
    2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
    3. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
    4. Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398.
    5. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
    6. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  • Entrez Gene IDs:
    1010 (Human)
  • Alternate Names:
    Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); Cadherin12; Cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma.
    Authors: Utsunomiya T, Sonoda H, Mimori K
    Clin. Cancer Res., 2004;10(17):5702-7.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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