Detects human CaM Kinase II gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) CaM Kinase II beta is observed, and less than 5% cross-reactivity with rhCaM Kinase II alpha and rhCaM Kinase II δ is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human CaM Kinase II gamma Ala448-Gln558 Accession # Q13555
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CaM Kinase II gamma by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CaM Kinase II gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase II gamma at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CaM Kinase II gamma
Calcium/calmodulin-dependent protein kinase type II gamma (CaMKII gamma ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. The widely expressed CaMKII gamma from human, mouse, and rat share 100% aa sequence identity within aa 448-558 of isoform 1.
Calcium/calmodulin-dependent Protein Kinase II gamma Subunit
Entrez Gene IDs:
818 (Human); 12325 (Mouse); 171140 (Rat)
calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II subunit gamma; CAMK; CAMK2G; CAMKG; CAMKGFLJ16043; CaMK-II subunit gamma; CAMK-II; EC 2.7.11; EC 220.127.116.11; MGC26678
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