Human Cannabinoid R2/CB2/CNR2 Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Cannabinoid R2/CB2/CNR2 in direct ELISAs. Stains human Cannabinoid R2/CB2/CNR2 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 352110
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human embryonic kidney cell line HEK293-derived transfected with human Cannabinoid R2/CB2/CNR2
    Met1-Cys360
    Accession # NP_001832
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Cannabinoid R2/CB2/CNR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Cannabinoid R2/CB2/CNR2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB36551R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Cannabinoid R2/CB2/CNR2
Cannabinoid R2 (Cannabinoid Receptor 2), also called CB2 or CNR2, is a 7TM protein that belongs to the family of G protein-coupled receptors, class A. Cannabinoid R2 is expressed in the periphery, notably by hematopoietic cells, in contrast to Cannabinoid R1 which is expressed predominantly in the central nervous system and gut. Cannabinoid R2 on lymphocytes and macrophages binds cannabinoids and modulates cytokine secretion. With the exception of their divergent C‑terminal tails, human Cannabinoid R2 shares 82% amino acid sequence identity with mouse and rat Cannabinoid R2.
  • Long Name:
    Cannabinoid Receptor 2
  • Entrez Gene IDs:
    1269 (Human); 12802 (Mouse); 57302 (Rat)
  • Alternate Names:
    Cannabinoid R2; cannabinoid receptor 2 (macrophage); CannabinoidR2; CB-2; CB2cannabinoid receptor 2; CB2R; CNR2; CX5; CX5testis-dominant CNR2 isoform CB2; hCB2
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