Home / Cannabinoid R2/CB2/CNR2 / Human Cannabinoid R2/CB2/CNR2 Antibody

Human Cannabinoid R2/CB2/CNR2 Antibody

Catalog #: MAB36551
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB36551-100
MAB36551-SP

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Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry.
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Citations (2)
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Human Cannabinoid R2/CB2/CNR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cannabinoid R2/CB2/CNR2 in direct ELISAs. Stains human Cannabinoid R2/CB2/CNR2 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 352110
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human Cannabinoid R2/CB2/CNR2
Met1-Cys360
Accession # NP_001832
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Cannabinoid R2/CB2/CNR2 antibody in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP antibody by Flow Cytometry. View Larger

Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Cannabinoid R2/CB2/ CNR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Cannabinoid R2/CB2/CNR2 Monoclonal Antibody (Catalog # MAB36551) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cannabinoid R2/CB2/CNR2

Cannabinoid R2 (Cannabinoid Receptor 2), also called CB2 or CNR2, is a 7TM protein that belongs to the family of G protein-coupled receptors, class A. Cannabinoid R2 is expressed in the periphery, notably by hematopoietic cells, in contrast to Cannabinoid R1 which is expressed predominantly in the central nervous system and gut. Cannabinoid R2 on lymphocytes and macrophages binds cannabinoids and modulates cytokine secretion. With the exception of their divergent C‑terminal tails, human Cannabinoid R2 shares 82% amino acid sequence identity with mouse and rat Cannabinoid R2.

Long Name
Cannabinoid Receptor 2
Entrez Gene IDs
1269 (Human); 12802 (Mouse); 57302 (Rat)
Alternate Names
Cannabinoid R2; cannabinoid receptor 2 (macrophage); CannabinoidR2; CB-2; CB2cannabinoid receptor 2; CB2R; CNR2; CX5; CX5testis-dominant CNR2 isoform CB2; hCB2

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Citations for Human Cannabinoid R2/CB2/CNR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture
    Authors: A Yeliseev, A van den Be, L Zoubak, K Hines, S Stepnowski, K Williston, W Yan, K Gawrisch, J Zmuda
    Sci Rep, 2020-10-08;10(1):16805.
    Species: E. coli
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inhibition of fatty acid amide hydrolase prevents pathology in neurovisceral acid sphingomyelinase deficiency by rescuing defective endocannabinoid signaling
    Authors: A Bartoll, A Toledano-Z, J Casas, M Guzmán, EH Schuchman, MD Ledesma
    EMBO Mol Med, 2020-10-05;0(0):e11776.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot

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