Human Carbohydrate Sulfotransferase 3/CHST3 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB7450AFP680

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 799011
View all Carbohydrate Sulfotransferase 3/CHST3 Primary Antibodies »

Product Specifications

Specificity

Detects human Carbohydrate Sulfotransferase 3/CHST3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1 or recombinant mouse CHST3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbohydrate Sulfotransferase 3/CHST3

The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).

References

  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.
  4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.
  5. Yusa, A. et al. (2006) J. Biol. Chem. 281: 20393.
  6. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

C6ST1, CHST3, GST-0

Entrez Gene IDs

9469 (Human); 53374 (Mouse); 84468 (Rat)

Gene Symbol

CHST3

UniProt

Q7LGC8

Additional Carbohydrate Sulfotransferase 3/CHST3 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail