Carbohydrate Sulfotransferase 3/CHST3 in Human Pancreas.
Carbohydrate Sulfotransferase 3/CHST3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Carbohydrate Sulfotransferase 3/CHST3 Monoclonal Antibody (Catalog # MAB7450) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbohydrate Sulfotransferase 3/CHST3
The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).
Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.
Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.
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