Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 799011
View all Carbohydrate Sulfotransferase 3/CHST3 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 3/CHST3
Glu39-Thr479
Accession # Q7LGC8

Specificity

Detects human Carbohydrate Sulfotransferase 3/CHST3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1 or recombinant mouse CHST3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Carbohydrate Sulfotransferase 3/CHST3 Antibody

Carbohydrate Sulfotransferase 3/CHST3 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Carbohydrate Sulfotransferase 3/CHST3 in Human Pancreas.

Carbohydrate Sulfotransferase 3/CHST3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Carbohydrate Sulfotransferase 3/CHST3 Monoclonal Antibody (Catalog # MAB7450) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Carbohydrate Sulfotransferase 3/CHST3 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbohydrate Sulfotransferase 3/CHST3

The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).

References

  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.
  4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.
  5. Yusa, A. et al. (2006) J. Biol. Chem. 281: 20393.
  6. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

C6ST1, CHST3, GST-0

Entrez Gene IDs

9469 (Human); 53374 (Mouse); 84468 (Rat)

Gene Symbol

CHST3

UniProt

Q7LGC8

Additional Carbohydrate Sulfotransferase 3/CHST3 Products

Product Documents for Human Carbohydrate Sulfotransferase 3/CHST3 Antibody

Certificate of Analysis

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Product Specific Notices for Human Carbohydrate Sulfotransferase 3/CHST3 Antibody

For research use only

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Protocols

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail