Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Carboxypeptidase A2/CPA2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
Tyr17-His417
Accession # P48052

Specificity

Detects human Carboxypeptidase A2/CPA2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) CPA1, rhCPB1, and recombinant mouse CPA4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Carboxypeptidase A2/CPA2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxypeptidase A2/CPA2

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417). The purified rhCPA2 corresponds to the pro form, which can be activated and assayed.

References

  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

Alternate Names

CPA2

Entrez Gene IDs

1358 (Human)

Gene Symbol

CPA2

UniProt

P48052

Additional Carboxypeptidase A2/CPA2 Products

Product Documents for Human Carboxypeptidase A2/CPA2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carboxypeptidase A2/CPA2 Biotinylated Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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