Human Caspr1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB7548V Datasheet / COA / SDS

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FAB7548V-100UG

All Caspr1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspr1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caspr1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Caspr2 is observed.

Source

Monoclonal Mouse IgG2a Clone # 753232

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Caspr1
Trp20-Gly1280
Accession # P78357

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Caspr1

Contactin-associated protein 1 (Caspr1), also known as Neurexin-4, Paranodin, and NCP1, is an approximately 190 kDa transmembrane protein that is concentrated at paranodal junctions of axons during myelination. It is required for junction formation and ion channel localization and plays an important role in the propagation of saltatory nerve conduction. Caspr1 interacts with Contactins and Nogo-A at the paranodal junction and inhibits the binding of Contactin to glial cell Neurofascin. Caspr1 is proteolytically cleaved by Reelin, and this action is inhibited by the cellular prion protein (PrP). Mature human Caspr1 consists of a 1264 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 80 aa cytoplasmic domain. The ECD contains one Factor 5/8-like domain, four Laminin-G-like domains, two EGF-like domains, and one Fibrinogen-like domain. Within the ECD, human Caspr1 shares 95% aa sequence identity with mouse and rat Caspr1.

Long Name

Contactin Associated Protein-like 1

Entrez Gene IDs

8506 (Human); 53321 (Mouse)

Alternate Names

Caspr; Caspr1; CASPRcaspr1; CNTNAP; CNTNAP1; contactin associated protein 1; neurexin 4; Neurexin IV; neurexin-4; NRXN4contactin-associated protein 1; p190