Human CCL11/Eotaxin PE‑conjugated Antibody

CCL11, also known as Eotaxin, is a secreted potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 is an O-glycosylated, 13 kDa peptide whose cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate a 74 amino acid residue mature human CCL11. At the amino acid sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. CCL11 circulates as a monomer, homodimer and heterodimer in a complex with either CCL2 or CCL8. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptors 3 and 5, plus CXCR3 have now been identified to be specific receptors for human CCL11. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibits infection by the HIV isolates.