Human CCL17/TARC Alexa Fluor® 594-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27 or recombinant rat CCL20 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 54015
  • Immunogen
    E. coli-derived recombinant human CCL17/TARC
    Ala24-Ser94
    Accession # Q92583.1
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL17/TARC

CCL17, also called TARC, is a member of the CC chemokine family. CCL17 cDNA encodes a highly basic 94 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24‑29% amino acid sequence identity with CCL1, 2, 3, 4, 5, 7 and 8. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

  • References:
    1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
    2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
    3. Nomiyama, H. et al. (1997) Genomics 40:211.
  • Entrez Gene IDs:
    6361 (Human); 20295 (Mouse)
  • Alternate Names:
    ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
Related Research Areas

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