CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
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Human CCL2/MCP-1 Quantikine ELISA Kit
R&D Systems | Catalog # DCP00
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Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
Sensitivity
10 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
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Product Summary for Human CCL2/MCP-1 Quantikine ELISA Kit
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human MCP-1 in this assay. No medical histories were available for the donors used
in this study. The reported urine values are actual and are not normalized for creatinine
content.
Cell Culture Supernates - Human peripheral blood leukocytes were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 2 or 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human MCP-1.
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serum (n=37) | 370 | 200-722 |
| EDTA plasma (n=37) | 153 | 72-295 |
| Citrate plasma (n=37) | 196 | 134-436 |
| Heparin plasma (n=37) | 242 | 113-340 |
| Urine (n=37) | 211 | 42-410 |
Cell Culture Supernates - Human peripheral blood leukocytes were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 2 or 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human MCP-1.
| Condition | Day 2 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 647 | 1785 |
| Stimulated | 67,225 | 70,000 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 78.2 | 360 | 1079 | 76.0 | 360 | 1086 |
| Standard Deviation | 3.3 | 17.7 | 63.5 | 4.5 | 17.2 | 48.5 |
| CV% | 4.2 | 4.9 | 5.9 | 5.9 | 4.8 | 4.5 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 76.7 | 364 | 1121 | 74.2 | 352 | 1076 |
| Standard Deviation | 6.0 | 17.1 | 54.4 | 5.0 | 20.5 | 49.4 |
| CV% | 7.8 | 4.7 | 4.9 | 6.7 | 5.8 | 4.6 |
Recovery for Human CCL2/MCP-1 Quantikine ELISA Kit
The recovery of MCP-1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 96 | 88-107 |
| Citrate Plasma (n=5) | 100 | 94-107 |
| EDTA Plasma (n=5) | 96 | 92-102 |
| Heparin Plasma (n=5) | 102 | 94-114 |
| Serum (n=5) | 103 | 92-113 |
| Urine (n=5) | 92 | 85-100 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MCP-1 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL2/MCP-1 Quantikine ELISA Kit
Human MCP-1 ELISA/CCL2 ELISA Cell Culture Supernate/Urine Standard Curve
Human MCP-1 ELISA/CCL2 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for Human CCL2/MCP-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL2/MCP-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human CCL2/MCP-1 Quantikine ELISA Kit
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Customer Reviews for Human CCL2/MCP-1 Quantikine ELISA Kit (12)
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12 Customer Ratings
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Sample Tested: HUVEC human umbilical vein endothelial cellsSpecies: HumanVerified Customer | Posted 01/13/2026MCP-1 ELISAWe detect MCP-1 from human endothelial cell supernatant
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Sample Tested: PlasmaVerified Customer | Posted 11/15/2023
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Sample Tested: Aortic smooth muscle cellsVerified Customer | Posted 07/25/2023
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Sample Tested: Cell culture supernatantVerified Customer | Posted 04/24/2023
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Sample Tested: UrineVerified Customer | Posted 03/15/2022Urine was tested at 2-fold, 4-fold, and 8-fold dilutions. All samples were in range at 2-fold dilution, 75% were in range at 4-fold dilution, and 62.5% were in range at 8-fold dilution. All samples at all dilutions had a %Recovery within 80-120% of the reference (2-fold) dilution. We continue to use this kit regularly.
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Sample Tested: Cell culture supernatantVerified Customer | Posted 11/05/2018
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Sample Tested: HAECVerified Customer | Posted 11/02/2018
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Sample Tested: Cell Culture MediaVerified Customer | Posted 05/01/2018
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Sample Tested: Primary human lunf fibroblast and Primary human lung fibroblastVerified Customer | Posted 12/04/2017This result has been published in the PlosONE journal: 10.1371/journal.pone.0058258
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Sample Tested: Human tissueVerified Customer | Posted 08/18/2017Very decent price and perfect customer support
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Sample Tested: cell culture supernatantsVerified Customer | Posted 07/24/2017cell culture supernatants were diluted 1:5
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Sample Tested: EDTA PlasmaVerified Customer | Posted 12/14/2016MCP-1 concentrations were measured in plasma of HIV+ subjects on HAART and in healthy controls. Blood was collected in EDTA-tubes and plasma separated by centrifugation at 2000rpm for 20 minutes at 20oC. Plasma was diluted 1:2 before use in the assay. Figure Legend for Figure: a) Standard curve for MCP-1 from which values were extrapolated. b) MCP-1 concentrations in plasma of HIV+ and HIV- subjects. Values are expressed as pg/ml and are significantly elevated in HIV+?ART group. Analyses done using non parametric unpaired Mann Whitney U-test.
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Protocols
View specific protocols for Human CCL2/MCP-1 Quantikine ELISA Kit (DCP00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.
- Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well.
- For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
For Cell Culture Supernate & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour. - Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent for Serum & Plasma Samples Only
200 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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