Human CCL5/RANTES Quantikine ELISA Kit

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SRN00B
PDRN00B
DRN00B
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Human CCL5/RANTES ELISA Platelet-Poor Plasma Standard Curve
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Citations (76)
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Human CCL5/RANTES Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sensitivity
6.6 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human RANTES
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human RANTES Immunoassay is a 3.5 hour solid phase ELISA designed to measure human RANTES levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human RANTES and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RANTES showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human RANTES.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 91.9 573 1120 92 511 1105
Standard Deviation 2.3 10 32.4 6.2 32.7 70.7
CV% 2.5 1.7 2.9 6.7 6.4 6.4

EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 108 599 1191 88 494 1063
Standard Deviation 3.9 14.3 41.5 9.1 43.1 89.6
CV% 3.6 2.4 3.5 10.3 8.7 8.4

Recovery

The recovery of RANTES spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 95-110
Platelet-poor Citrate Plasma (n=4) 99 93-105
Platelet-poor EDTA Plasma (n=4) 98 93-103
Platelet-poor Heparin Plasma (n=4) 99 93-105
Serum (n=5) 101 94-107
Urine (n=10) 102 88-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RANTES were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL5/RANTES ELISA Linearity

Data Examples

Human CCL5/RANTES ELISA Platelet-Poor Plasma Standard Curve

Human CCL5/RANTES ELISA Cell Culture Supernates/Serum/Urine Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL5/RANTES

CCL5/RANTES is a secreted beta-chemokine that plays a primary role in the inflammatory immune response through chemoattraction and activation of leukocytes that express CCR1, 3, 4, or 5. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1.

Entrez Gene IDs:
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names:
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CCL5/RANTES Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. Induction of chemokine (C-C motif) ligand 5 by Epstein-Barr virus infection enhances tumor angiogenesis in nasopharyngeal carcinoma
    Authors: W Ma, L Feng, S Zhang, H Zhang, X Zhang, X Qi, Y Zhang, Q Feng, T Xiang, YX Zeng
    Cancer Sci., 2018;109(5):1710-1722.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Suppression of STING associated with LKB1 loss in KRAS-driven lung cancer
    Authors: S Kitajima, E Ivanova, S Guo, R Yoshida, M Campisi, SK Sundararam, S Tange, Y Mitsuishi, TC Thai, S Masuda, BP Piel, LM Sholl, PT Kirschmeie, CP Paweletz, H Watanabe, M Yajima, DA Barbie
    Cancer Discov, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
    Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
    Cancer Sci., 2018;109(3):724-731.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017;13(6):4995-5001.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Tumor necrosis factor alpha inhibitors have no effect on a human T-lymphotropic virus type-I (HTLV-I)-infected cell line from patients with HTLV-I-associated myelopathy
    Authors: S Fukui, H Nakamura, Y Takahashi, N Iwamoto, H Hasegawa, K Yanagihara, T Nakamura, A Okayama, A Kawakami
    BMC Immunol, 2017;18(1):7.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF-?B/STAT3 pathways
    Authors: A Wang, F Zhang, H Xu, M Xu, Y Cao, C Wang, Y Xu, M Su, M Zhang, Y Zhuge
    Mol. Immunol., 2017;87(0):67-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
    Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
    Sci Rep, 2017;7(0):42893.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus
    Authors: ZS Guo, Z Liu, M Sathaiah, J Wang, R Ravindrana, E Kim, S Huang, TW Kenniston, JC Bell, HJ Zeh, LH Butterfiel, A Gambotto, DL Bartlett
    Mol Ther Methods Clin Dev, 2017;7(0):112-122.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
    Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
    Oncotarget, 2017;8(54):92134-92142.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017;15(1):121.
    Species: Human
    Sample Types: Plasma
  11. -2518 A/G MCP-1 but not -403 G/A RANTES gene polymorphism is associated with enhanced risk of basal cell carcinoma
    Authors: Roman Nowicki
    Postepy Dermatol Alergol, 2016;33(5):381-385.
    Species: Human
    Sample Types: Serum
  12. Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
    Cytokine, 2016;84(0):17-24.
    Species: Human
    Sample Types: Plasma
  13. Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro.
    Authors: Bader A, Klose K, Bieback K, Korinth D, Schneider M, Seifert M, Choi Y, Kurtz A, Falk V, Stamm C
    PLoS ONE, 2015;10(9):e0138477.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Loss of intraepidermal nerve fiber density during SIV peripheral neuropathy is mediated by monocyte activation and elevated monocyte chemotactic proteins.
    Authors: Lakritz J, Robinson J, Polydefkis M, Miller A, Burdo T
    J Neuroinflammation, 2015;12(0):237.
    Species: Primate
    Sample Types: Plasma
  15. Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma.
    Authors: Sadeghi M, Lahdou I, Oweira H, Daniel V, Terness P, Schmidt J, Weiss K, Longerich T, Schemmer P, Opelz G, Mehrabi A
    Br J Cancer, 2015;113(5):756-62.
    Species: Human
    Sample Types: Serum
  16. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015;195(5):2429-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Chlorogenic acid inhibits human platelet activation and thrombus formation.
    Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
    PLoS ONE, 2014;9(3):e90699.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Nuclear NF-kappaB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.
    Authors: Yi B, Hu X, Zhang H, Huang J, Liu J, Hu J, Li W, Huang L
    PLoS ONE, 2014;9(6):e99633.
    Species: Human
    Sample Types: Urine
  19. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2014;164(2):286-95.
    Species: Human
    Sample Types: Serum
  20. IL-10 up-regulates CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats.
    Authors: Kim H, Kim H
    Cytokine, 2014;68(1):40-9.
    Species: Rat
    Sample Types: Cell Culture Supernates
  21. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
    J. Thromb. Haemost., 2012;10(8):1616-23.
    Species: Human
    Sample Types: Serum
  22. Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
    Authors: de-Oliveira-Pinto LM, Marinho CF, Povoa TF, de Azeredo EL, de Souza LA, Barbosa LD, Motta-Castro AR, Alves AM, Avila CA, de Souza LJ, da Cunha RV, Damasco PV, Paes MV, Kubelka CF
    PLoS ONE, 2012;7(7):e38527.
    Species: Human
    Sample Types: Plasma
  23. Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
    Authors: Landro L, Ueland T, Otterdal K, Froland SS, Aukrust P
    J. Thromb. Haemost., 2011;9(5):1075-7.
    Species: Human
    Sample Types: Platelet Poor Plasma
  24. A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
    Authors: Su Y, Amiri KI, Horton LW
    Clin. Cancer Res., 2010;16(1):348-57.
    Species: Human
    Sample Types: Serum
  25. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009;30(4):313-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
    Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
    Biochem. Biophys. Res. Commun., 2009;378(1):90-4.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. RANTES in exhaled breath condensate of stable and unstable asthma patients.
    Authors: Zietkowski Z, Tomasiak MM, Skiepko R, Bodzenta-Lukaszyk A
    Respir Med, 2008;102(8):1198-202.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)
  30. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
    Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
    J. Immunol., 2008;181(9):5857-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
    Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
    Int. J. Cancer, 2008;122(4):769-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
    Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
    Cancer Res., 2008;68(23):9996-10003.
    Species: Human
    Sample Types: Serum
  33. Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
    Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
    Life Sci., 2008;82(23):1203-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
    Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
    Biochem. Pharmacol., 2008;76(1):79-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
    Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
    J. Interferon Cytokine Res., 2008;28(5):297-310.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Differential chemokine expression in tubular cells in response to urinary proteins from patients with nephrotic syndrome.
    Authors: Huang Z, Wen Q, Zhou SF, Yu XQ
    Cytokine, 2008;42(2):222-33.
    Species: Human
    Sample Types: Urine
  38. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007;111(1):175-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
    Authors: Emad A, Emad Y
    J. Clin. Immunol., 2007;27(6):605-12.
    Species: Human
    Sample Types: BALF
  40. High T-cell response to human cytomegalovirus induces chemokine-mediated endothelial cell damage.
    Authors: Bolovan-Fritts CA, Trout RN, Spector SA
    Blood, 2007;110(6):1857-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
    Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
    Am. J. Respir. Crit. Care Med., 2007;177(1):44-55.
    Species: Human
    Sample Types: BALF
  42. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  43. Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
    Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
    J. Immunol., 2007;178(12):7678-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Circulating interleukin-15 and RANTES chemokine in Parkinson's disease.
    Authors: Rentzos M, Nikolaou C, Andreadou E, Paraskevas GP, Rombos A, Zoga M, Tsoutsou A, Boufidou F, Kapaki E, Vassilopoulos D
    Acta Neurol. Scand., 2007;116(6):374-9.
    Species: Human
    Sample Types: Serum
  45. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007;111(4):2339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
    Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
    Nature, 2007;449(7162):557-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
    Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
    Chest, 2007;133(1):26-33.
    Species: Human
    Sample Types: Saliva
  48. Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
    Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
    J. Immunol., 2006;176(6):3788-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
    Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
    Int. Immunol., 2006;18(8):1243-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 in follicular fluid accumulate differentially in patients with and without endometriosis undergoing in vitro fertilization.
    Authors: Schultze-Mosgau A, Agic A, Diedrich K, Hornung D
    Fertil. Steril., 2006;86(6):1616-20.
    Species: Human
    Sample Types: Serum
  53. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
  54. Interaction between the CCR5 chemokine receptors and microbial HSP70.
    Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
    Eur. J. Immunol., 2006;36(9):2304-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. RANTES and monocyte chemoattractant protein 1 as sensitive markers of disease activity in patients with juvenile rheumatoid arthritis: a six-year longitudinal study.
    Authors: Yao TC, Kuo ML, See LC, Ou LS, Lee WI, Chan CK, Huang JL
    Arthritis Rheum., 2006;54(8):2585-93.
    Species: Human
    Sample Types: Serum
  56. Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
    Authors: Bartosik-Psujek H, Stelmasiak Z
    J Neural Transm, 2005;112(6):797-803.
    Species: Human
    Sample Types: Serum
  57. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Types: Serum
  58. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Types: Serum
  59. Airway inflammation during stable and acutely exacerbated chronic obstructive pulmonary disease.
    Authors: Fujimoto K, Yasuo M, Urushibata K, Hanaoka M, Koizumi T, Kubo K
    Eur. Respir. J., 2005;25(4):640-6.
    Species: Human
    Sample Types: Sputum
  60. Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
    Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
    J. Gen. Virol., 2004;85(0):2603-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
    Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
    J. Exp. Med., 2004;199(9):1213-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
    Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
    Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
    Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
    Transplantation, 2004;78(9):1341-50.
    Species: Human
    Sample Types: Urine
  64. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
    Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
    J. Virol., 2004;78(12):6151-61.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Types: Cell Culture Supernates
  65. Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
    Authors: Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R
    , 2002;294(1):209-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection.
    Authors: Burton CT, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N
    Clin. Exp. Immunol., 2002;130(2):286-92.
    Species: Human
    Sample Types: Plasma
  67. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
    Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
    J. Immunol., 2002;169(8):4172-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
    Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
    Eur. J. Immunol., 2002;32(7):2037-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites.
    Authors: Milliken D, Scotton C, Raju S, Balkwill F, Wilson J
    Clin. Cancer Res., 2002;8(4):1108-14.
    Species: Human
    Sample Types: Serum
  70. CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
    Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
    Blood, 2002;100(7):2321-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
    Authors: Sato E, Koyama S
    Eur. Respir. J., 2000;16(5):951-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Up-regulation of beta-chemokines and down-modulation of CCR5 co-receptors inhibit simian immunodeficiency virus transmission in non-human primates.
    Authors: Lehner T, Wang Y, Cranage M, Tao L, Mitchell E, Bravery C, Doyle C, Pratt K, Hall G, Dennis M, Villinger L, Bergmeier L
    Immunology, 2000;99(4):569-77.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  73. Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.
    Authors: Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP
    Proc. Natl. Acad. Sci. U.S.A., 1999;96(24):14031-6.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  74. Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
    Authors: Lehner T, Wang Y, Cranage M, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R
    Nat. Med., 1996;2(7):767-75.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  75. Human CD4- 8- T cells are a distinctive immunoregulatory subset.
    Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
    FASEB J, 0;24(7):2558-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

  1. Can plasma be used as a sample in Human CCL5/RANTES Quantikine® ELISA kit (Catalog # DRN00B) if platelet-poor plasma is not available?  

    • Platelet-poor plasma is the only type of plasma sample validated for Human CCL5/RANTES Quantikine® ELISA kit. CCL5/RANTES is present in platelet granules and is released upon platelet activation. 

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Human CCL5/RANTES Quantikine ELISA Kit
By Anonymous on 03/21/2019
Sample Tested: Brain Microvascular Endothelial Cells

Human CCL5/RANTES Quantikine ELISA Kit
By Anonymous on 01/09/2018
Sample Tested: EDTA Plasma,Citrate Plasma,Heparin Plasma