Human CCL5/RANTES Quantikine ELISA Kit

(68 citations)   
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
  • Sensitivity
    6.6 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
  • Specificity
    Natural and recombinant human RANTES
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC20, Quantikine Immunoassay Control Group 3 - Please Inquire
Product Summary
The Quantikine Human RANTES Immunoassay is a 3.5 hour solid phase ELISA designed to measure human RANTES levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human RANTES and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RANTES showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human RANTES.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean91.95731120925111105
Standard Deviation2.31032.46.232.770.7
CV%2.51.72.96.76.46.4

EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1085991191884941063
Standard Deviation3.914.341.59.143.189.6
CV%3.62.43.510.38.78.4

Recovery

The recovery of RANTES spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 95-110
Platelet-poor Citrate Plasma (n=4) 99 93-105
Platelet-poor EDTA Plasma (n=4) 98 93-103
Platelet-poor Heparin Plasma (n=4) 99 93-105
Serum (n=5) 101 94-107
Urine (n=10) 102 88-114
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RANTES were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL5/RANTES Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL5/RANTES
CCL5/RANTES is a secreted beta-chemokine that plays a primary role in the inflammatory immune response through chemoattraction and activation of leukocytes that express CCR1, 3, 4, or 5. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1.
    • Entrez Gene IDs
      6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline);
    • Alternate Names
      CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    68 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
      Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
      J Transl Med, 2017;15(1):121.
      Species: Human
      Sample Type: Plasma
    2. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
      Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
      Oncol Lett, 2017;13(6):4995-5001.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
      Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
      Sci Rep, 2017;7(0):42893.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Tumor necrosis factor alpha inhibitors have no effect on a human T-lymphotropic virus type-I (HTLV-I)-infected cell line from patients with HTLV-I-associated myelopathy
      Authors: S Fukui, H Nakamura, Y Takahashi, N Iwamoto, H Hasegawa, K Yanagihara, T Nakamura, A Okayama, A Kawakami
      BMC Immunol, 2017;18(1):7.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
      Cytokine, 2016;84(0):17-24.
      Species: Human
      Sample Type: Plasma
    6. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
      Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
      J Immunol, 2015;195(5):2429-41.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Loss of intraepidermal nerve fiber density during SIV peripheral neuropathy is mediated by monocyte activation and elevated monocyte chemotactic proteins.
      Authors: Lakritz J, Robinson J, Polydefkis M, Miller A, Burdo T
      J Neuroinflammation, 2015;12(0):237.
      Species: Primate
      Sample Type: Plasma
    8. Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma.
      Authors: Sadeghi M, Lahdou I, Oweira H, Daniel V, Terness P, Schmidt J, Weiss K, Longerich T, Schemmer P, Opelz G, Mehrabi A
      Br J Cancer, 2015;113(5):756-62.
      Species: Human
      Sample Type: Serum
    9. Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro.
      Authors: Bader A, Klose K, Bieback K, Korinth D, Schneider M, Seifert M, Choi Y, Kurtz A, Falk V, Stamm C
      PLoS ONE, 2015;10(9):e0138477.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
      Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
      Br J Haematol, 2014;164(2):286-95.
      Species: Human
      Sample Type: Serum
    11. IL-10 up-regulates CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats.
      Authors: Kim H, Kim H
      Cytokine, 2014;68(1):40-9.
      Species: Rat
      Sample Type: Cell Culture Supernates
    12. Chlorogenic acid inhibits human platelet activation and thrombus formation.
      Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
      PLoS ONE, 2014;9(3):e90699.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Nuclear NF-kappaB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.
      Authors: Yi B, Hu X, Zhang H, Huang J, Liu J, Hu J, Li W, Huang L
      PLoS ONE, 2014;9(6):e99633.
      Species: Human
      Sample Type: Urine
    14. Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
      Authors: de-Oliveira-Pinto LM, Marinho CF, Povoa TF, de Azeredo EL, de Souza LA, Barbosa LD, Motta-Castro AR, Alves AM, Avila CA, de Souza LJ, da Cunha RV, Damasco PV, Paes MV, Kubelka CF
      PLoS ONE, 2012;7(7):e38527.
      Species: Human
      Sample Type: Plasma
    15. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
      J. Thromb. Haemost., 2012;10(8):1616-23.
      Species: Human
      Sample Type: Serum
    16. Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
      Authors: Landro L, Ueland T, Otterdal K, Froland SS, Aukrust P
      J. Thromb. Haemost., 2011;9(5):1075-7.
      Species: Human
      Sample Type: Platelet-Poor Plasma
    17. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
      Authors: Tso GH, Law HK, Tu W
      Stem Cells, 2010;28(5):939-54.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Human CD4- 8- T cells are a distinctive immunoregulatory subset.
      Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
      FASEB J., 2010;24(7):2558-66.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
      Authors: Su Y, Amiri KI, Horton LW
      Clin. Cancer Res., 2010;16(1):348-57.
      Species: Human
      Sample Type: Serum
    20. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
      Authors: Renaud SJ, Sullivan R, Graham CH
      Placenta, 2009;30(4):313-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
      Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
      J. Exp. Med., 2009;206(1):169-81.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
      Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
      Biochem. Biophys. Res. Commun., 2009;378(1):90-4.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
      Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
      Cancer Res., 2008;68(23):9996-10003.
      Species: Human
      Sample Type: Serum
    24. Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
      Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
      Life Sci., 2008;82(23):1203-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
      Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
      Int. J. Cancer, 2008;122(4):769-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. RANTES in exhaled breath condensate of stable and unstable asthma patients.
      Authors: Zietkowski Z, Tomasiak MM, Skiepko R, Bodzenta-Lukaszyk A
      Respir Med, 2008;102(8):1198-202.
      Species: Human
      Sample Type: Exhaled Breath Condensate (EBC)
    27. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
      Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
      J. Interferon Cytokine Res., 2008;28(5):297-310.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
      Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
      J. Neuroimmunol., 2008;199(1):35-45.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Differential chemokine expression in tubular cells in response to urinary proteins from patients with nephrotic syndrome.
      Authors: Huang Z, Wen Q, Zhou SF, Yu XQ
      Cytokine, 2008;42(2):222-33.
      Species: Human
      Sample Type: Urine
    30. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
      Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
      J. Immunol., 2008;181(9):5857-64.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
      Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
      Biochem. Pharmacol., 2008;76(1):79-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    32. Circulating interleukin-15 and RANTES chemokine in Parkinson's disease.
      Authors: Rentzos M, Nikolaou C, Andreadou E, Paraskevas GP, Rombos A, Zoga M, Tsoutsou A, Boufidou F, Kapaki E, Vassilopoulos D
      Acta Neurol. Scand., 2007;116(6):374-9.
      Species: Human
      Sample Type: Serum
    33. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
      Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
      Am. J. Respir. Crit. Care Med., 2007;177(1):44-55.
      Species: Human
      Sample Type: BALF
    34. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
      Authors: Bolovan-Fritts CA, Spector SA
      Blood, 2007;111(1):175-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    35. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
      Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
      Blood, 2007;111(4):2339-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    36. High T-cell response to human cytomegalovirus induces chemokine-mediated endothelial cell damage.
      Authors: Bolovan-Fritts CA, Trout RN, Spector SA
      Blood, 2007;110(6):1857-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
      Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
      J. Allergy Clin. Immunol., 2007;120(5):1118-25.
      Species: Human
      Sample Type: BALF
    38. Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
      Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
      J. Immunol., 2007;178(12):7678-85.
      Species: Human
      Sample Type: Cell Culture Supernates
    39. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
      Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
      Nature, 2007;449(7162):557-63.
      Species: Human
      Sample Type: Cell Culture Supernates
    40. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
      Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
      Chest, 2007;133(1):26-33.
      Species: Human
      Sample Type: Saliva
    41. Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
      Authors: Emad A, Emad Y
      J. Clin. Immunol., 2007;27(6):605-12.
      Species: Human
      Sample Type: BALF
    42. Regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 in follicular fluid accumulate differentially in patients with and without endometriosis undergoing in vitro fertilization.
      Authors: Schultze-Mosgau A, Agic A, Diedrich K, Hornung D
      Fertil. Steril., 2006;86(6):1616-20.
      Species: Human
      Sample Type: Serum
    43. Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
      Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
      Int. Immunol., 2006;18(8):1243-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    44. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
      Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
      J. Am. Coll. Cardiol., 2006;48(8):1591-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    45. Interaction between the CCR5 chemokine receptors and microbial HSP70.
      Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
      Eur. J. Immunol., 2006;36(9):2304-14.
      Species: Human
      Sample Type: Cell Culture Supernates
    46. Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
      Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
      J. Immunol., 2006;176(6):3788-95.
      Species: Human
      Sample Type: Cell Culture Supernates
    47. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
      Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
      J. Exp. Med., 2006;203(12):2763-77.
      Species: Human
      Sample Type: Cell Culture Supernates
    48. RANTES and monocyte chemoattractant protein 1 as sensitive markers of disease activity in patients with juvenile rheumatoid arthritis: a six-year longitudinal study.
      Authors: Yao TC, Kuo ML, See LC, Ou LS, Lee WI, Chan CK, Huang JL
      Arthritis Rheum., 2006;54(8):2585-93.
      Species: Human
      Sample Type: Serum
    49. Increased ENA-78 in the follicular fluid of patients with endometriosis.
      Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
      Acta Obstet Gynecol Scand, 2006;85(3):336-42.
      Species: Human
      Sample Type: Follicular Fluid
    50. Airway inflammation during stable and acutely exacerbated chronic obstructive pulmonary disease.
      Authors: Fujimoto K, Yasuo M, Urushibata K, Hanaoka M, Koizumi T, Kubo K
      Eur. Respir. J., 2005;25(4):640-6.
      Species: Human
      Sample Type: Sputum
    51. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    52. Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
      Authors: Bartosik-Psujek H, Stelmasiak Z
      J Neural Transm, 2005;112(6):797-803.
      Species: Human
      Sample Type: Serum
    53. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
      Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
      Microbes Infect., 2005;7(3):427-36.
      Species: Human
      Sample Type: Serum
    54. Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
      Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
      J. Gen. Virol., 2004;85(0):2603-13.
      Species: Human
      Sample Type: Cell Culture Supernates
    55. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
      Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
      Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    56. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
      Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
      Transplantation, 2004;78(9):1341-50.
      Species: Human
      Sample Type: Urine
    57. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
      Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
      J. Exp. Med., 2004;199(9):1213-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    58. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
      Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
      J. Virol., 2004;78(12):6151-61.
      Species: Primate - Pan troglodytes (Chimpanzee)
      Sample Type: Cell Culture Supernates
    59. Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection.
      Authors: Burton CT, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N
      Clin. Exp. Immunol., 2002;130(2):286-92.
      Species: Human
      Sample Type: Plasma
    60. Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
      Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
      Virology, 2002;294(1):209-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    61. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
      Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
      J. Immunol., 2002;169(8):4172-82.
      Species: Human
      Sample Type: Cell Culture Supernates
    62. Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites.
      Authors: Milliken D, Scotton C, Raju S, Balkwill F, Wilson J
      Clin. Cancer Res., 2002;8(4):1108-14.
      Species: Human
      Sample Type: Serum
    63. CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
      Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
      Blood, 2002;100(7):2321-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    64. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
      Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
      Eur. J. Immunol., 2002;32(7):2037-45.
      Species: Human
      Sample Type: Cell Culture Supernates
    65. Up-regulation of beta-chemokines and down-modulation of CCR5 co-receptors inhibit simian immunodeficiency virus transmission in non-human primates.
      Authors: Lehner T, Wang Y, Cranage M, Tao L, Mitchell E, Bravery C, Doyle C, Pratt K, Hall G, Dennis M, Villinger L, Bergmeier L
      Immunology, 2000;99(4):569-77.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Cell Culture Supernates
    66. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
      Authors: Sato E, Koyama S
      Eur. Respir. J., 2000;16(5):951-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    67. Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.
      Authors: Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP
      Proc. Natl. Acad. Sci. U.S.A., 1999;96(24):14031-6.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Cell Culture Supernates
    68. Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
      Authors: Lehner T, Wang Y, Cranage M, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R
      Nat. Med., 1996;2(7):767-75.
      Species: Primate - Macaca mulatta (Rhesus Macaque)
      Sample Type: Cell Culture Supernates
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    Quantikine Immunoassay Control Group 3

    Ctrl QC20
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Have you used Human CCL5/RANTES Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    FAQs

    1. Can plasma be used as a sample in Human CCL5/RANTES Quantikine® ELISA kit (Catalog # DRN00B) if platelet-poor plasma is not available?  

      • Platelet-poor plasma is the only type of plasma sample validated for Human CCL5/RANTES Quantikine® ELISA kit. CCL5/RANTES is present in platelet granules and is released upon platelet activation. 

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