Human CCL7/MCP-3 Quantikine ELISA Kit

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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    8.52 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human MCP-3
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human MCP-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MCP-3 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human MCP-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-3.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation5.9410.720.210.219.937.7

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation6.248.3518.16.8714.224.3


The recovery of MCP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 92-115
EDTA Plasma (n=5) 101 88-111
Heparin Plasma (n=5) 98 85-114
Serum (n=5) 94 86-110
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MCP-3 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL7/MCP-3 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL7/MCP-3/MARC
Human MCP-3 and mouse MARC are members of the CC subfamily of chemokines and are monocyte chemoattractants. Sequence comparisons suggest that MARC may be the mouse homolog of the human MCP-3 gene. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene.
    • Entrez Gene IDs
      6354 (Human); 20306 (Mouse);
    • Alternate Names
      C-C motif chemokine 7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3);
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 9 of 9
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    Sample Type
    1. Bindarit inhibits human coronary artery smooth muscle cell proliferation, migration and phenotypic switching.
      Authors: Maddaluno M, Grassia G, Di Lauro M, Parisi A, Maione F, Cicala C, De Filippis D, Iuvone T, Guglielmotti A, Maffia P, Mascolo N, Ialenti A
      PLoS ONE, 2012;7(10):e47464.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
      Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
      Int. J. Cancer, 2010;127(2):332-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
      Authors: Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA
      Proteomics, 2008;8(11):2211-9.
      Species: Human
      Sample Type: Serum
    4. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
      Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
      J. Immunol., 2007;178(2):1172-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
      Authors: Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA
      J. Immunol., 2007;178(3):1948-59.
      Species: Human
      Sample Type: BALF
    6. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
      Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
      Blood, 2006;108(3):947-55.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
      Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
      J. Virol., 2004;78(12):6151-61.
      Species: Primate - Pan troglodytes (Chimpanzee)
      Sample Type: Cell Culture Supernates
    8. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
      Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
      Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
      Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
      Eur. J. Immunol., 2002;32(7):2037-45.
      Species: Human
      Sample Type: Cell Culture Supernates
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 839 for Human CCL7/MCP-3

    Ctrl QC119
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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