Human CCL7/MCP-3 Quantikine ELISA Kit

R&D Systems | Catalog # DCC700

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

8.52 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all CCL7/MCP-3/MARC ELISA Kits »

Product Summary for Human CCL7/MCP-3 Quantikine ELISA Kit

The Quantikine Human MCP-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MCP-3 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human MCP-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-3.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human MCP-3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 136 274 506 138 277 524
Standard Deviation 6.24 8.35 18.1 6.87 14.2 24.3
CV% 4.6 3.0 3.6 5.0 5.1 4.6

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 140 278 560 152 297 575
Standard Deviation 5.94 10.7 20.2 10.2 19.9 37.7
CV% 4.2 3.8 3.6 6.7 6.7 6.6

Recovery for Human CCL7/MCP-3 Quantikine ELISA Kit

The recovery of MCP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 92-115
EDTA Plasma (n=5) 101 88-111
Heparin Plasma (n=5) 98 85-114
Serum (n=5) 94 86-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MCP-3 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CCL7/MCP-3/MARC ELISA Linearity

Scientific Data Images for Human CCL7/MCP-3 Quantikine ELISA Kit

Human CCL7/MCP-3/MARC ELISA Cell Culture Supernate Standard Curve

Human CCL7/MCP-3/MARC ELISA Cell Culture Supernate Standard Curve

Human CCL7/MCP-3/MARC ELISA Serum/Plasma Standard Curve

Human CCL7/MCP-3/MARC ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL7/MCP-3/MARC

Human MCP-3 and mouse MARC are members of the CC subfamily of chemokines and are monocyte chemoattractants. Sequence comparisons suggest that MARC may be the mouse homolog of the human MCP-3 gene. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene.

Alternate Names

MARC, MCP-3

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Gene Symbol

CCL7

Additional CCL7/MCP-3/MARC Products

Product Documents for Human CCL7/MCP-3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCL7/MCP-3 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CCL7/MCP-3 Quantikine ELISA Kit

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  • Human CCL7/MCP-3 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 04/26/2022
    Measured urine & plasma at 2-fold dilution from healthy controls & patients with disease. All samples for both biofluids were below the standard curve.
    Human CCL7/MCP-3 Quantikine ELISA Kit DCC700
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

View specific protocols for Human CCL7/MCP-3 Quantikine ELISA Kit (DCC700):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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