Human CCR1 Alexa Fluor® 488-conjugated Antibody

Clone 53504 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB145G-100UG
Detection of CCR1 in Human PBMCs by Flow Cytometry.
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Human CCR1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry. No cross‑reactivity with CCR5 transfectants is observed.
Source
Monoclonal Mouse IgG2B Clone # 53504
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Swiss 3T3 mouse cell line transfected with human CCR1
Accession # P32246
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR1 in Human PBMCs by Flow Cytometry. View Larger

Detection of CCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB145G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR1

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Entrez Gene IDs
1230 (Human); 12768 (Mouse); 57301 (Rat)
Alternate Names
C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R

Product Datasheets

FAQs

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