Human CCR6 Alexa Fluor® 594-conjugated Antibody

Clone 53103 was used by HLDA to establish CD designation.
  • Species Reactivity
  • Specificity
    Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
  • Source
    Monoclonal Mouse IgG2B Clone # 53103
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR6
    Accession # P51684
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CCR6 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB195T) or (B) Mouse IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC0041T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR6

Human CCR6 (C-C Chemokine Receptor 6) is a 45-50 kDa member of the G-protein coupled receptor #1 family. It is a 374 amino acid (aa) 7-transmembrane glyocprotein that is responsive to a limited number of ligands, including CCL20/MIP-3 alpha and the beta -Defensins HBD-1 and -2. A large number of cell types express CCR6, which in human include neutrophils, spermatogonia, intestinal epithelial columnar cells, osteoblasts, IL-17 secreting CD161+ CCR6+ TCR-V alpha 7.2 CD8+ Tcells, monocytes, macrophages, CD4+ CCR4+ CXCR3- CCR6+ IL-17 secreting Th17 cells, CD4+ CCR4- CXCR3+ CCR6+ IL-17 secreting and IFN-gamma secreting Th1 Th17 cells and hepatic carcinoma plus colon carcinoma cells. On mouse cells, CCR6 is found on CD4+ CD25+ CD45RO+ memory Tregs, CD4- CD8- CD25- CD44+ c-Kit+ CCR6+ double-negative thymocytes, and CD116+ CCR6+ dendritic cells plus CD11c+ B220+ CD19+ IgM+ CCR6+ Peyer's Patch B cells. CCR6 activity may be cell or environment specific, and actions described to date include the promotion of cell proliferation, the downregulation of IL-12 production, and chemotaxis. The extracellular domains of human and mouse CCR6 share 52% aa sequence identity.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC0041T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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