Human CCR6 Alexa Fluor® 594-conjugated Antibody

Clone 53103 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB195T-100
Detection  of CCR6 in Human PBMCs by Flow Cytometry.
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Human CCR6 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
Source
Monoclonal Mouse IgG2B Clone # 53103
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR6
Met1-Met374
Accession # P51684
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CCR6 in Human PBMCs by Flow Cytometry. View Larger

Detection of CCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CCR6 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB195T) or (B) Mouse IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC0041T). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

Human CCR6 (C-C Chemokine Receptor 6) is a 45-50 kDa member of the G-protein coupled receptor #1 family. It is a 374 amino acid (aa) 7-transmembrane glyocprotein that is responsive to a limited number of ligands, including CCL20/MIP-3 alpha and the beta -Defensins HBD-1 and -2. A large number of cell types express CCR6, which in human include neutrophils, spermatogonia, intestinal epithelial columnar cells, osteoblasts, IL-17 secreting CD161+ CCR6+ TCR-V alpha 7.2 CD8+ Tcells, monocytes, macrophages, CD4+ CCR4+ CXCR3- CCR6+ IL-17 secreting Th17 cells, CD4+ CCR4- CXCR3+ CCR6+ IL-17 secreting and IFN-gamma secreting Th1 Th17 cells and hepatic carcinoma plus colon carcinoma cells. On mouse cells, CCR6 is found on CD4+ CD25+ CD45RO+ memory Tregs, CD4- CD8- CD25- CD44+ c-Kit+ CCR6+ double-negative thymocytes, and CD116+ CCR6+ dendritic cells plus CD11c+ B220+ CD19+ IgM+ CCR6+ Peyer's Patch B cells. CCR6 activity may be cell or environment specific, and actions described to date include the promotion of cell proliferation, the downregulation of IL-12 production, and chemotaxis. The extracellular domains of human and mouse CCR6 share 52% aa sequence identity.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

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