Human CCR6 Antibody

Clone 53103 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB195-100
MAB195-SP
MAB195-500
CCR6 in Human Spleen.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (1)

Human CCR6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
Source
Monoclonal Mouse IgG2B Clone # 53103
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR6
Met1-Met374
Accession # P51684
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood CD19+ B cells
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CCR6 in Human Spleen. View Larger

CCR6 in Human Spleen. CCR6 was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Human CCR6 Monoclonal Antibody (Catalog # MAB195) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

Product Datasheets

Citations for Human CCR6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. The Cerebellum of Patients with Steatohepatitis Shows Lymphocyte Infiltration, Microglial Activation and Loss of Purkinje and Granular Neurons
    Authors: T Balzano, J Forteza, P Molina, J Giner, A Monzó, J Sancho-Jim, A Urios, C Montoliu, V Felipo
    Sci Rep, 2018;8(1):3004.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. CCR6 is required for ligand-induced CatSper activation in human sperm
    Authors: R Diao, T Wang, KL Fok, X Li, Y Ruan, MK Yu, Y Cheng, Y Chen, H Chen, L Mou, X Cai, Y Wang, Z Cai, X Zeng, HC Chan
    Oncotarget, 2017;8(53):91445-91458.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma
    Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang
    Oncol Lett, 2017;14(6):8183-8189.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm
    Authors: B Soto, T Gallastegi, C Rodríguez, J Martínez-G, JR Escudero, L Vila, M Camacho
    Sci Rep, 2017;7(1):17331.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis
    Authors: W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia?
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  6. Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset
    Authors: J Dunham, N van Driel, BJ Eggen, C Paul, BA 't Hart, JD Laman, YS Kap
    Clin Transl Immunology, 2017;6(2):e127.
    Species: Primate
    Sample Types: Whole Cells
    Applications: Flow
  7. CCR6 expression in colon cancer is associated with advanced disease and supports epithelial-to-mesenchymal transition
    Br J Cancer, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016;0(0):.
    Species: Rhesus Macaque
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. CCR6, the sole receptor for the chemokine CCL20, promotes spontaneous intestinal tumorigenesis.
    Authors: Nandi, Bisweswa, Pai, Christin, Huang, Qin, Prabhala, Rao H, Munshi, Nikhil C, Gold, Jason S
    PLoS ONE, 2014;9(5):e97566.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  10. Increased Th17/Treg ratio in chronic liver GVHD.
    Authors: Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier J, Moreau P, Gregoire M, Gaugler B, Mohty M
    Bone Marrow Transplant, 2014;49(4):539-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  11. Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression.
    Authors: Schanz A, Red-Horse K, Hess A, Baston-Bust D, Heiss C, Krussel J
    Placenta, 2014;35(12):1089-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  12. CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.
    Authors: Liu, Jinlin, Ke, Fang, Xu, Zhenyao, Liu, Zhaoyuan, Zhang, Lingyun, Yan, Sha, Wang, Zhe, Wang, Hong, Wang, Honglin
    PLoS ONE, 2014;9(6):e101137.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Increased plasmacytoid dendritic cells and RORgammat-expressing immune effectors in cutaneous acute graft-versus-host disease.
    Authors: Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier J, Moreau P, Gregoire M, Gaugler B, Mohty M
    J Leukoc Biol, 2013;94(6):1337-43.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  14. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013;131(4):1146-56, 1156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  15. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  16. CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells.
    Authors: Kim S, Lewis C, Nadel JA
    J. Immunol., 2011;186(6):3392-400.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB
  17. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  18. Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
    Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
    J. Invest. Dermatol., 2010;130(6):1646-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  19. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  20. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  21. Characterisation of dendritic cell subsets in lung cancer micro-environments.
    Authors: Bergeron A, El-Hage F, Kambouchner M, Lecossier D, Tazi A
    Eur. Respir. J., 2006;28(6):1170-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
    Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
    J. Immunol., 2006;176(10):5735-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  23. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
    Authors: Page G, Miossec P
    J. Pathol., 2004;204(1):28-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  25. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004;164(2):409-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  27. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  28. CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells.
    Authors: Maki W, Morales RE, Carroll VA, Telford WG, Knibbs RN, Stoolman LM, Hwang ST
    J. Immunol., 2002;169(5):2346-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
    Authors: Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E
    J Immunol, 0;166(11):6477-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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Immunohistochemistry Reagents

Isotype Controls

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Secondary Antibodies

Mouse IgG2B Antibody

Reviews for Human CCR6 Antibody

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Human CCR6 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Plasma Species: Human