Human CCR6 Antibody

Clone 53103 was used by HLDA to establish CD designation.
(22 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
  • Source
    Monoclonal Mouse IgG2B Clone # 53103
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR6
    Met1-Met374
    Accession # P51684
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood CD19+ B cells
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CCR6 in Human Spleen. CCR6 was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Human CCR6 Monoclonal Antibody (Catalog # MAB195) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis
    Authors: W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia?
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CCR6 expression in colon cancer is associated with advanced disease and supports epithelial-to-mesenchymal transition
    Br J Cancer, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.
    Authors: Liu, Jinlin, Ke, Fang, Xu, Zhenyao, Liu, Zhaoyuan, Zhang, Lingyun, Yan, Sha, Wang, Zhe, Wang, Hong, Wang, Honglin
    PLoS ONE, 2014;9(6):e101137.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression.
    Authors: Schanz A, Red-Horse K, Hess A, Baston-Bust D, Heiss C, Krussel J
    Placenta, 2014;35(12):1089-94.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. CCR6, the sole receptor for the chemokine CCL20, promotes spontaneous intestinal tumorigenesis.
    Authors: Nandi, Bisweswa, Pai, Christin, Huang, Qin, Prabhala, Rao H, Munshi, Nikhil C, Gold, Jason S
    PLoS ONE, 2014;9(5):e97566.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Increased Th17/Treg ratio in chronic liver GVHD.
    Authors: Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier J, Moreau P, Gregoire M, Gaugler B, Mohty M
    Bone Marrow Transplant, 2014;49(4):539-44.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013;131(4):1146-56, 1156.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Increased plasmacytoid dendritic cells and RORgammat-expressing immune effectors in cutaneous acute graft-versus-host disease.
    Authors: Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier J, Moreau P, Gregoire M, Gaugler B, Mohty M
    J Leukoc Biol, 2013;94(6):1337-43.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells.
    Authors: Kim S, Lewis C, Nadel JA
    J. Immunol., 2011;186(6):3392-400.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  12. Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
    Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
    J. Invest. Dermatol., 2010;130(6):1646-56.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Characterisation of dendritic cell subsets in lung cancer micro-environments.
    Authors: Bergeron A, El-Hage F, Kambouchner M, Lecossier D, Tazi A
    Eur. Respir. J., 2006;28(6):1170-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  16. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
    Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
    J. Immunol., 2006;176(10):5735-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  17. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  18. Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
    Authors: Page G, Miossec P
    J. Pathol., 2004;204(1):28-38.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004;164(2):409-17.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  21. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  22. CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells.
    Authors: Maki W, Morales RE, Carroll VA, Telford WG, Knibbs RN, Stoolman LM, Hwang ST
    J. Immunol., 2002;169(5):2346-53.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 22 Citations
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