Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Bioassay, Blocking

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 53103
View all CCR6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CCR6
Met1-Met374
Accession # P51684

Specificity

Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCR6 Antibody

CCR6 antibody in Human Spleen by Immunohistochemistry (IHC-P).

CCR6 in Human Spleen.

CCR6 was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Human CCR6 Monoclonal Antibody (Catalog # MAB195) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CCR6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood CD19+ B cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Reviewed Applications

Read 3 reviews rated 4.3 using MAB195 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Alternate Names

CC-CKR-6, CCR6, CD196, CKR-L3, CKRL3, CMKBR6, DRY6, GPR-CY4, GPR29, GPRCY4, STRL22

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Gene Symbol

CCR6

UniProt

P51684

Additional CCR6 Products

Product Documents for Human CCR6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCR6 Antibody

For research use only

Citations for Human CCR6 Antibody

Customer Reviews for Human CCR6 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human CCR6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: LCH cells
    Species: Human
    Verified Customer | Posted 08/13/2021
    Human CCR6 Antibody MAB195
  • Human CCR6 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human CCR6 Antibody MAB195
  • Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/08/2018

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Protocols

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FAQs

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