Human CD1c/BDCA‑1 APC‑conjugated Antibody

Detection of CD1c/BDCA‑1 in MOLT‑4 Human Cell Line by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Goat Anti-Human CD1c/BDCA-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5910A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Human CD1c/BDCA‑1 by Flow Cytometry

Endotoxin sensitivity correlates with CD14 expression.A Expression of TLR4 and CD14 on different human immune cells. Cells were plated and induced as described in Figure 3. After 24 h, cells were stained for the expression of TLR4 and CD14 and analysed by flow cytometry. Results show the percentage of fluorescence-positive cells (% pos cells) compared to the respective isotype controls. Statistical analysis was performed as described in Figure 3. B Analysis of CD1c, CD14 and CD19 expression on primary human CD1c+ DCs. 1×105 CD1c+ DCs were stained for the expression of CD1c, CD14 or CD19 either directly after isolation (0 h), or after 24 h culture in 24-well plates containing 1 ml of DC-medium. Cells were then analysed by flow cytometry. One representative donor is shown. Light grey, isotype control; dark grey, respective surface marker staining. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25478795), licensed under a CC-BY license. Not internally tested by R&D Systems.