Human CD20 (Research Grade Rituximab Biosimilar) Alexa Fluor® 405-conjugated Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
FAB9575V-100UG
Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry.
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Human CD20 (Research Grade Rituximab Biosimilar) Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu2
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CD20
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MS4A1/CD20 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry. Human PBMC were stained with (A) Human Anti-Human CD20 (Research Grade Rituximab Biosimilar) Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB9575V) or (B) unstained, and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. This antibody can be used in flow cytometry or various assay formats to measure CD20 expression.

Long Name
Cluster of Differentiation 20
Entrez Gene IDs
931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)
Alternate Names
B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

Product Datasheets

FAQs

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