Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
FAB9575B-100
Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu2
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CD20
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MS4A1/CD20 antibody in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP antibody by Flow Cytometry. View Larger

Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry. HEK293 Human cell line transfected with (A) Human MS4A1/CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human MS4A1/CD20 (Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD20 antibody in Daudi Human Cell Line by Immunocytochemistry (ICC). View Larger

CD20 in Daudi Human Cell Line. CD20 was detected in unfixed live Daudi human Burkitt's lymphoma cell line using Human Anti-Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. The biotinylated antibody can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.

Long Name
Cluster of Differentiation 20
Entrez Gene IDs
931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)
Alternate Names
B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

Product Datasheets

You must select a language.

x

Product Specific Notices

Rituximab is a biosimliar expressed in HEK293 cells and is intended for research use only.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody and earn rewards!

Have you used Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review