Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody

R&D Systems | Catalog # FAB9575B

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu2
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

Human CD20

Specificity

Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images

Detection of MS4A1/CD20 antibody in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP antibody by Flow Cytometry.

Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.

HEK293 Human cell line transfected with (A) Human MS4A1/CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human MS4A1/CD20 (Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
CD20 antibody in Daudi Human Cell Line by Immunocytochemistry (ICC).

CD20 in Daudi Human Cell Line.

CD20 was detected in unfixed live Daudi human Burkitt's lymphoma cell line using Human Anti-Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Unfixed live Daudi human Burkitt's lymphoma cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. The biotinylated antibody can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

Rituximab is a biosimliar expressed in HEK293 cells and is intended for research use only.

For research use only

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Protocols

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FAQs

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