Human CD229/SLAMF3 Alexa Fluor® 350-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD229/SLAMF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse CD229 is observed.
  • Source
    Monoclonal Mouse IgG2a Clone # 249936
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD229/SLAMF3
    Lys48-Lys454
    Accession # Q9HBG7
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 350
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    Human whole blood lymphocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD229/SLAMF3

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig‑like V-set and two Ig‑like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). The ECD of human CD229 shares 57%‑59% aa sequence identity with mouse and rat CD229. Within the first two Ig‑like domains that are common to all SLAM proteins, human CD229 shares 24%‑39% aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig‑like domain or short cytoplasmic region (2). CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells (2‑6). Homophilic binding between CD229 molecules is mediated by the N-terminal Ig‑like domain (7). Human and mouse CD229 exhibit cross-species binding (7). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell‑B cell contact (7). Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization (8, 9). In addition, there are two ITSMs which mediate phosphorylation-dependent CD229 association with SAP and SHP-2 (10). These four tyrosine-containing motifs are intact in the described CD229 splice variants. CD229 knockout mice show minimally impaired immune responses, suggesting functional redundancy with other molecules (11).

  • References:
    1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
    2. de la Fuente, M.A. et al. (2001) Blood 97:3513. 
    3. Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.
    4. Romero, X. et al. (2004) Tissue Antigens 64:132. 
    5. Hogarth, P.M. et al. (1980) Immunogenetics 11:65. 
    6. Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.
    7. Romero, X. et al. (2005) J. Immunol. 174:7033.
    8. Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.
    9. Martin, M. et al. (2005) J. Immunol. 174:5977.
    10. Sayos, J. et al. (2001) Blood 97:3867.
    11. Graham, D.B. et al. (2006) J. Immunol. 176:291.
  • Long Name:
    SLAM Family Member 3
  • Entrez Gene IDs:
    4063 (Human); 17085 (Mouse)
  • Alternate Names:
    CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9
Related Research Areas
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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