Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 249936
View all CD229/SLAMF3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD229/SLAMF3
Lys48-Lys454
Accession # Q9HBG7

Specificity

Detects human CD229/SLAMF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse CD229 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD229/SLAMF3 APC‑conjugated Antibody

Detection of CD229/SLAMF3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD229/SLAMF3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD229/SLAMF3 APC-conjugated Monoclonal Antibody (Catalog # FAB1898A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD229/SLAMF3 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 1 review rated 4 using FAB1898A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD229/SLAMF3

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig‑like V-set and two Ig‑like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). The ECD of human CD229 shares 57%‑59% aa sequence identity with mouse and rat CD229. Within the first two Ig‑like domains that are common to all SLAM proteins, human CD229 shares 24%‑39% aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig‑like domain or short cytoplasmic region (2). CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells (2‑6). Homophilic binding between CD229 molecules is mediated by the N-terminal Ig‑like domain (7). Human and mouse CD229 exhibit cross-species binding (7). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell‑B cell contact (7). Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization (8, 9). In addition, there are two ITSMs which mediate phosphorylation-dependent CD229 association with SAP and SHP-2 (10). These four tyrosine-containing motifs are intact in the described CD229 splice variants. CD229 knockout mice show minimally impaired immune responses, suggesting functional redundancy with other molecules (11).

References

  1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
  2. de la Fuente, M.A. et al. (2001) Blood 97:3513. 
  3. Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.
  4. Romero, X. et al. (2004) Tissue Antigens 64:132. 
  5. Hogarth, P.M. et al. (1980) Immunogenetics 11:65. 
  6. Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.
  7. Romero, X. et al. (2005) J. Immunol. 174:7033.
  8. Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.
  9. Martin, M. et al. (2005) J. Immunol. 174:5977.
  10. Sayos, J. et al. (2001) Blood 97:3867.
  11. Graham, D.B. et al. (2006) J. Immunol. 176:291.

Long Name

SLAM Family Member 3

Alternate Names

CD229, Ly9, SLAMF3

Entrez Gene IDs

4063 (Human); 17085 (Mouse)

Gene Symbol

LY9

UniProt

Q9HBG7

Additional CD229/SLAMF3 Products

Product Documents for Human CD229/SLAMF3 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human CD229/SLAMF3 APC‑conjugated Antibody

For research use only

Customer Reviews for Human CD229/SLAMF3 APC‑conjugated Antibody (1)

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  • Human CD229/SLAMF3 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte and monocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: 2% Human Serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human CD229/SLAMF3 APC‑conjugated Antibody FAB1898A

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