The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Human CD3+ T Cell Enrichment Column, Small, Pack of 5
R&D Systems | Catalog # HTCC-5
Key Product Details
Species
Human
Product Summary for Human CD3+ T Cell Enrichment Column, Small, Pack of 5
T Cell Enrichment Columns
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| View Full Assay Principle |
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.
CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 37% and 54% of initial CD3+ cells loaded. The purity of recovered cells ranges from 87% to 95%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.
Features
- Available for human, mouse, and rat cells
- Purifies CD3+ T cells to 87 – 95%
- Based upon gravitational flow
- Simple procedure
- No specialized equipment required
Kit Contents
- Cell Selection Column
- 10X Column Buffer
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Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD3
Alternate Names
CD_antigen: CD3e, CD3 antigen, delta subunit, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, CD3G, CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T3DOKT3, delta chain, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE
Gene Symbol
CD3E
Additional CD3 Products
Product Documents for Human CD3+ T Cell Enrichment Column, Small, Pack of 5
Certificate of Analysis
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Product Specific Notices for Human CD3+ T Cell Enrichment Column, Small, Pack of 5
For research use only
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Citations for Human CD3+ T Cell Enrichment Column, Small, Pack of 5
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