MagCellect Human CD3+ T Cell Isolation Kit Summary
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Citations for MagCellect Human CD3+ T Cell Isolation Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Exosomes from red blood cell units bind to monocytes and induce proinflammatory cytokines, boosting T-cell responses in vitro.
Authors: Danesh A, Inglis H, Jackman R, Wu S, Deng X, Muench M, Heitman J, Norris P
Blood, 2014;123(5):687-96. 2014
Mitogenic effect contributes to increased virulence of Streptococcus suis sequence type 7 to cause streptococcal toxic shock-like syndrome.
Authors: Zheng H, Ye C, Segura M, Gottschalk M, Xu J
Clin. Exp. Immunol., 2008;153(3):385-91. 2008
WIP is a chaperone for Wiskott-Aldrich syndrome protein (WASP).
Authors: de la Fuente MA, Sasahara Y, Calamito M, Anton IM, Elkhal A, Gallego MD, Suresh K, Siminovitch K, Ochs HD, Anderson KC, Rosen FS, Geha RS, Ramesh N
Proc. Natl. Acad. Sci. U.S.A., 2007;104(3):926-31. 2007
Transient regulatory T-cells: a state attained by all activated human T-cells.
Authors: Pillai V, Ortega SB, Wang CK, Karandikar NJ
Clin. Immunol., 2007;123(1):18-29. 2007
When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?
It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.
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