Human CD300e/LMIR6 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects mouse CD300e/LMIR6 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD300e/LMIR6
    Gly20-Leu169
    Accession # BAG60333
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human LMIR6/CD300LE
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood monocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD300e/LMIR6
The CD300, alternately designated the LMIR family, contains six to eight type I membrane proteins expressed mainly on cells of the myeloid lineage. Each has a single extracellular V-type Ig-like domain. Some have cytoplasmic ITIM motifs that result in the delivery of an inhibitory signal; others can interact with ITAM motifs and function as activating receptors. Human CD300 proteins have also been designated IREM (immune receptor expressed by myeloid cells) or CMRF-35 for the original identifying antibody; mouse CD300 proteins have alternate naming as CLM (CMRF-35-like molecules).
  • Entrez Gene IDs:
    342510 (Human); 217306 (Mouse)
  • Alternate Names:
    CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2
Related Research Areas
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