Detection of CD302/CLEC13A in Human Blood Monocytes by Flow Cytometry.Human peripheral blood monocytes were stained with Mouse Anti-Human CD302/CLEC13A Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6367G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
CD302, also known as DCL-1 and CLEC13A, is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to the fusion protein. There is one potential alternative start site at Met63. Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.