Human CD302/CLEC13A Antibody

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human CD302/CLEC13A in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse CD302 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 771910
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD302/CLEC13A
    Asp23-Asn167
    Accession # Q81X05
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD302/CLEC13A in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD302/
CLEC13A Monoclonal Antibody (Catalog # MAB6367, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD302/CLEC13A

CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein.  It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205.  CD302 contributes aa 23-232 to the fusion protein.  There is one potential alternative start site at Met63.  Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.

  • Entrez Gene IDs:
    9936 (Human); 66205 (Mouse); 295629 (Rat)
  • Alternate Names:
    BIMLEC; CD302 antigenC-type lectin domain family 13, member A; CD302 molecule; CD302; CLEC13A; CLEC13AMGC22301; C-type lectin domain family 13 member A; DCL1; DCL-1; DCL1C-type lectin BIMLEC; KIAA0022FLJ43091; Type I transmembrane C-type lectin receptor DCL-1
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 148  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG1 Isotype Control

Ctrl MAB002R 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human CD302/CLEC13A Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee