CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to the fusion protein. There is one potential alternative start site at Met63. Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 771910
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD302/CLEC13A
Asp23-Asn167
Accession # Q81X05
Asp23-Asn167
Accession # Q81X05
Specificity
Detects human CD302/CLEC13A in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with
recombinant mouse CD302 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD302/CLEC13A Antibody
Detection of CD302/CLEC13A in Human Monocytes by Flow Cytometry.
Human PBMC were stained with (A) Mouse Anti-Human CD302/CLEC13A Monoclonal Antibody (Catalog # MAB6367) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and PE-conjugated Mouse anti-Human CD14 Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD302/CLEC13A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD302/CLEC13A
Alternate Names
BIMLEC, CD302, CLEC13A, DCL1
Gene Symbol
CD302
UniProt
Additional CD302/CLEC13A Products
Product Documents for Human CD302/CLEC13A Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CD302/CLEC13A Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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