Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 9G11
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD31/PECAM‑1 APC‑conjugated Antibody

Detection of CD31/PECAM-1 antibody in Human Granulocytes antibody by Flow Cytometry.

Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3567A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD31/PECAM-1 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

Detection of CD31/PECAM-1 in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3567A, filled histogram) or isotype control antibody (IC002A, open histogram). Staining was performed using our Membrane-Associated Staining protocol.
CD31/PECAM-1 Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

CD31/PECAM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

CD31/PECAM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3567A, filled histogram) or isotype control antibody (IC002A, open histogram). No staining in the CD31/PECAM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD31/PECAM‑1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes and THP-1 acute monocytic leukemia cell line

Knockout Validated

CD31/PECAM-1 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in CD31/PECAM-1 knockout THP-1 cell line

Reviewed Applications

Read 1 review rated 3 using FAB3567A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Human CD31/PECAM‑1 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD31/PECAM‑1 APC‑conjugated Antibody

For research use only

Citations for Human CD31/PECAM‑1 APC‑conjugated Antibody

Customer Reviews for Human CD31/PECAM‑1 APC‑conjugated Antibody (1)

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID: 23200853
    Species: Human
    Verified Customer | Posted 01/23/2015

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