Human CD31/PECAM-1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (4)
Supplemental Products

Human CD31/PECAM-1 Biotinylated Antibody Summary

Species Reactivity
Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human
(rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.
Monoclonal Mouse IgG1 Clone # 9G11
Protein A or G purified from ascites
Activated HUVEC human umbilical vein endothelial cells
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human CD31/PECAM‑1 (Catalog # ADP6) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
HUVEC human umbilical vein endothelial cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

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Citations for Human CD31/PECAM-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Comprehensive epigenome characterization reveals diverse transcriptional regulation across human vascular endothelial cells
    Authors: R Nakato, Y Wada, R Nakaki, G Nagae, Y Katou, S Tsutsumi, N Nakajima, H Fukuhara, A Iguchi, T Kohro, Y Kanki, Y Saito, M Kobayashi, A Izumi-Tagu, N Osato, K Tatsuno, A Kamio, Y Hayashi-Ta, H Wada, S Ohta, M Aikawa, H Nakajima, M Nakamura, RC McGee, KW Heppner, T Kawakatsu, M Genno, H Yanase, H Kume, T Senbonmats, Y Homma, S Nishimura, T Mitsuyama, H Aburatani, H Kimura, K Shirahige
    Epigenetics Chromatin, 2019;12(1):77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Purification
  2. SPIO labeling of endothelial cells using ultrasound and targeted microbubbles at diagnostic pressures
    Authors: I Skachkov, Y Luan, ST van Tiel, AFW van der St, N de Jong, MR Bernsen, K Kooiman
    PLoS ONE, 2018;13(9):e0204354.
    Species: Human, N/A
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, Labeling
  3. High-throughput flow cytometry screening of human hepatocellular carcinoma reveals CD146 to be a novel marker of tumor-initiating cells.
    Authors: Chen K, Ding A, Ding Y, Ghanekar A
    Biochem Biophys Rep, 0;8(0):107-113.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes.
    Authors: Brownell J, Wagoner J, Lovelace E, Thirstrup D, Mohar I, Smith W, Giugliano S, Li K, Crispe I, Rosen H, Polyak S
    J Hepatol, 0;59(4):701-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection


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