Human CD31/PECAM-1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3567P-100
FAB3567P-025
Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry.
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (2)

Human CD31/PECAM-1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 9G11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
CD31/PECAM-1 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in CD31/PECAM-1 knockout THP-1 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD31/PECAM-1 antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD31/PECAM-1 antibody in THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD31/PECAM-1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Membrane-Associated Staining protocol.

Knockout Validated CD31/PECAM-1  Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

CD31/PECAM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. CD31/PECAM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (IC002P, open histogram). No staining in the CD31/PECAM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

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Citations for Human CD31/PECAM-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Cancer-associated fibroblast-derived WNT2 increases tumor angiogenesis in colon cancer
    Authors: Daniela Unterleuthner, Patrick Neuhold, Katharina Schwarz, Lukas Janker, Benjamin Neuditschko, Harini Nivarthi et al.
    Angiogenesis
  2. GATA6 regulates anti-angiogenic properties in human cardiac fibroblasts via modulating LYPD1 expression
    Authors: Masuda, S;Matsuura, K;Shimizu, T;
    Regenerative therapy
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro
    Authors: Y Basmaeil, E Bahattab, A Al Subayyi, HB Kulayb, M Alrodayyan, M Abumaree, T Khatlani
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of a large-scale vascular bed for the in�vitro creation of three-dimensional cardiac tissue
    Authors: A Inui, H Sekine, K Sano, I Dobashi, A Yoshida, K Matsuura, E Kobayashi, M Ono, T Shimizu
    Regen Ther, 2019-10-22;11(0):316-323.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture
    Authors: S Masuda, K Matsuura, T Shimizu
    Regen Ther, 2018-07-07;9(0):1-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection, ICC
  6. Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix
    Authors: T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour
    Sci Rep, 2018-04-20;8(1):6331.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
    Authors: Norton, Kerri-An, Han, Zheyi, Popel, Aleksand, Pandey, Niranjan
    Onco Targets Ther, 2014-09-09;7(0):1571-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway.
    Authors: Kryza T, Achard C, Parent C, Marchand-Adam S, Guillon-Munos A, Iochmann S, Korkmaz B, Respaud R, Courty Y, Heuze-Vourc'h N
    FASEB J, 2013-11-13;28(2):740-51.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    2013-09-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Flow Cytometry
  10. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012-10-09;7(10):e47187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro
    Authors: Xiaoyu Xing, Zhiyuan Zhang, Liang Zhong, Guanqun Ju, Xiangyu Zou, Yingjian Zhu et al.
    Experimental and Therapeutic Medicine

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Reviews for Human CD31/PECAM-1 PE-conjugated Antibody

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Human CD31/PECAM-1 PE-conjugated Antibody
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human

Human CD31/PECAM-1 PE-conjugated Antibody
By Anonymous on 08/16/2016
Application: Flow Sample Tested: Cells from hematopoietic differentiation of hESC line H9 Species: Human