Human CD31/PECAM-1 PE-conjugated Antibody

(2 citations)
(1 Review)
  • Species Reactivity
  • Specificity
    Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 9G11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB3567P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

  • Long Name:
    Platelet Endothelial Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    5175 (Human); 18613 (Mouse); 29583 (Rat)
  • Alternate Names:
    adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012;7(10):e47187.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Flow Human Anonymous 08/16/2016


ApplicationFlow Cytometry
Sample TestedCells from hematopoietic differentiation of hESC line H9

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