Human CD31/PECAM-1 Antibody

Catalog # Availability Size / Price Qty
BBA7
Detection  of Human CD31/PECAM‑1 by Western Blot.
2 Images
Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (5)

Human CD31/PECAM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.
Source
Monoclonal Mouse IgG1 Clone # 9G11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human CD31/PECAM‑1 by Western Blot. View Larger

Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CD31/PECAM‑1 in HUVEC Human Cells. View Larger

CD31/PECAM‑1 in HUVEC Human Cells. CD31/PECAM‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule

Product Datasheets

Citations for Human CD31/PECAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain
    Authors: J Luzuriaga, O Pastor-Alo, JM Encinas, F Unda, G Ibarretxe, JR Pineda
    Front Physiol, 2019;10(0):347.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Straightforward method for singularized and region-specific CNS microvessels isolation
    Authors: J Rose Dayto, MC Franke, Y Yuan, L Cruz-Oreng
    J. Neurosci. Methods, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
    Authors: G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams
    PLoS ONE, 2018;13(10):e0204177.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
    Authors: K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms
    Authors: Lopa Leach
    Placenta, 2016;48(0):87-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis
    Sci Rep, 2016;6(0):29884.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling
    Stem Cell Res Ther, 2016;7(1):115.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  8. Endothelial Surface Protrusion by a Point Force
    Authors: Y Chen, L Lu, JY Shao
    Biophys. J, 2016;110(5):1150-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis.
    Authors: Weston, Chris J, Shepherd, Emma L, Claridge, Lee C, Rantakari, Pia, Curbishley, Stuart M, Tomlinson, Jeremy W, Hubscher, Stefan G, Reynolds, Gary M, Aalto, Kristiin, Anstee, Quentin, Jalkanen, Sirpa, Salmi, Marko, Smith, David J, Day, Christop, Adams, David H
    J Clin Invest, 2015;125(2):501-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014;68(9):1029-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
    Authors: Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava S, Koya D
    Diabetes, 2014;63(6):2120-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance.
    Authors: Martinelli R, Zeiger A, Whitfield M, Sciuto T, Dvorak A, Van Vliet K, Greenwood J, Carman C
    J Cell Sci, 2014;127(0):3720-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  13. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007;109(10):4264-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment.
    Authors: Williamson MR, Shuttleworth A, Canfield AE, Black RA, Kielty CM
    Biomaterials, 2007;28(35):5307-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Mechanisms of endothelial cell protection by blockade of the JAK2 pathway.
    Authors: Neria F, Caramelo C, Peinado H, Gonzalez-Pacheco FR, Deudero JJ, de Solis AJ, Fernandez-Sanchez R, Penate S, Cano A, Castilla MA
    Am. J. Physiol., Cell Physiol., 2006;292(3):C1123-31.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Disruption of graft endothelium correlates with early failure after off-pump coronary artery bypass surgery.
    Authors: Manchio JV, Gu J, Romar L, Brown J, Gammie J, Pierson RN, Griffith B, Poston RS
    Ann. Thorac. Surg., 2005;79(6):1991-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004;15(3):566-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization.
    Authors: Wei H, Song J, Fang L, Li G, Chatterjee S
    Biochem. Biophys. Res. Commun., 2004;320(4):1228-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Stimulation of primary human endothelial cell proliferation by IFN.
    Authors: Gomez D, Reich NC
    J. Immunol., 2003;170(11):5373-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human CD31/PECAM-1 Antibody

Average Rating: 4 (Based on 5 Reviews)

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Human CD31/PECAM-1 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human CD31/PECAM-1 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human CD31/PECAM-1 Antibody
By Anonymous on 02/28/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Antibody was reconstituted at 0.5mg/ml and used at 1:50 dilution overnight in 4ºC.


Human CD31/PECAM-1 Antibody
By Anonymous on 08/29/2016
Application: IHC Sample Tested: artery Species: Human

Frozen human artery. Permeabilized with 80% acetone.
Primary antibody at 1:100 incubate at 4oC overnight
Secondary antibody: CY3 donkey anti-mouse secondary antibody. 1 hour at room temperature.


Human CD31/PECAM-1 Antibody
By Anonymous on 08/29/2016
Application: IHC Sample Tested: Muscle tissue Species: Human

Antigen retrieval: 10mM Citrate pH6.
Primary antibody at 1:100 incubate at 4oC overnight
Secondary antibody: Alexa 647 donkey anti-mouse secondary antibody. 1 hour at room temperature.