Human CD31/PECAM-1 Antibody

(16 citations)
(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 9G11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunoprecipitation
    Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
CD31/PECAM‑1 in HUVEC Human Cells. CD31/PECAM‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1
The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.
  • Long Name:
    Platelet Endothelial Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    5175 (Human); 18613 (Mouse); 29583 (Rat)
  • Alternate Names:
    adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
    Authors: K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis
    Sci Rep, 2016;6(0):29884.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling
    Stem Cell Res Ther, 2016;7(1):115.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Paraffin embedded
  4. Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis.
    Authors: Weston, Chris J, Shepherd, Emma L, Claridge, Lee C, Rantakari, Pia, Curbishley, Stuart M, Tomlinson, Jeremy W, Hubscher, Stefan G, Reynolds, Gary M, Aalto, Kristiin, Anstee, Quentin, Jalkanen, Sirpa, Salmi, Marko, Smith, David J, Day, Christop, Adams, David H
    J Clin Invest, 2015;125(2):501-20.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  5. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014;68(9):1029-35.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  6. Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance.
    Authors: Martinelli R, Zeiger A, Whitfield M, Sciuto T, Dvorak A, Van Vliet K, Greenwood J, Carman C
    J Cell Sci, 2014;127(0):3720-34.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  7. Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
    Authors: Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava S, Koya D
    Diabetes, 2014;63(6):2120-31.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007;109(10):4264-71.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment.
    Authors: Williamson MR, Shuttleworth A, Canfield AE, Black RA, Kielty CM
    Biomaterials, 2007;28(35):5307-18.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Mechanisms of endothelial cell protection by blockade of the JAK2 pathway.
    Authors: Neria F, Caramelo C, Peinado H, Gonzalez-Pacheco FR, Deudero JJ, de Solis AJ, Fernandez-Sanchez R, Penate S, Cano A, Castilla MA
    Am. J. Physiol., Cell Physiol., 2006;292(3):C1123-31.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Disruption of graft endothelium correlates with early failure after off-pump coronary artery bypass surgery.
    Authors: Manchio JV, Gu J, Romar L, Brown J, Gammie J, Pierson RN, Griffith B, Poston RS
    Ann. Thorac. Surg., 2005;79(6):1991-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  12. Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization.
    Authors: Wei H, Song J, Fang L, Li G, Chatterjee S
    Biochem. Biophys. Res. Commun., 2004;320(4):1228-35.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004;167(2):377-88.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004;15(3):566-74.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004;167(2):377-88.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  16. Stimulation of primary human endothelial cell proliferation by IFN.
    Authors: Gomez D, Reich NC
    J. Immunol., 2003;170(11):5373-81.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 16 Citations
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Average Rating: 4.3 (Based on 3 reviews)

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We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human CD31/PECAM-1 Antibody BBA7
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Very Good
 ICC/IF Human Anonymous 02/28/2017
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Image Details
Immunocytochemistry/Immunofluorescence Human CD31/PECAM-1 Antibody BBA7
Immunocytochemistry/Immunofluorescence: Human CD31/PECAM-1 Antibody [BBA7]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedHUVEC human umbilical vein endothelial cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAntibody was reconstituted at 0.5mg/ml and used at 1:50 dilution overnight in 4ºC.
Immunohistochemistry Human CD31/PECAM-1 Antibody BBA7
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Very Good
 IHC Human Anonymous 08/29/2016
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Immunohistochemistry Human CD31/PECAM-1 Antibody BBA7
Immunohistochemistry: Human CD31/PECAM-1 Antibody [BBA7]

Summary

ApplicationImmunohistochemistry
Sample Testedartery
SpeciesHuman

Other Experimental Details

Other Experimental DetailsFrozen human artery. Permeabilized with 80% acetone.Primary antibody at 1:100 incubate at 4oC overnightSecondary antibody: CY3 donkey anti-mouse secondary antibody. 1 hour at room temperature.
Immunohistochemistry Human CD31/PECAM-1 Antibody BBA7
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Excellent
 IHC Human Anonymous 08/29/2016
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Immunohistochemistry Human CD31/PECAM-1 Antibody BBA7
Immunohistochemistry: Human CD31/PECAM-1 Antibody [BBA7]

Summary

ApplicationImmunohistochemistry
Sample TestedMuscle tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAntigen retrieval: 10mM Citrate pH6.Primary antibody at 1:100 incubate at 4oC overnightSecondary antibody: Alexa 647 donkey anti-mouse secondary antibody. 1 hour at room temperature.

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