Human CD63 Alexa Fluor® 488-conjugated Antibody Summary
Accession # P08962
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD63 in PBMC monocytes by Flow Cytometry. PBMC monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CD63 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5048G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.
Product Specific Notices
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Citation for Human CD63 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A novel approach to correlate the salivary exosomes and their protein cargo in the progression of cognitive impairment into Alzheimer's disease
Authors: K Rani, S Rastogi, P Vishwakarm, PS Bharti, V Sharma, K Renu, GP Modi, VY Vishnu, P Chatterjee, AB Dey, F Nikolajeff, S Kumar
J Neurosci Methods, 2020-10-17;347(0):108980.
Sample Types: Cell Lysates
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