Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 460305
View all CD63 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human CD63
Met1-Met238
Accession # P08962

Specificity

Stains human CD63 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD63 Antibody

Detection of CD63 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD63 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were stained with Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

CD63 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

CD63 in A549 Human Cell Line.

CD63 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of CD63 in PBMC monocytes by Flow Cytometry

PBMC monocytes were stained with Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD63 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMCs fixed with paraformaldehyde and permeabilized with saponin; Human PBMC monocytes (surface stain)

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB5048 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Entrez Gene IDs

967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)

Gene Symbol

CD63

UniProt

P08962

Additional CD63 Products

Product Documents for Human CD63 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD63 Antibody

For research use only

Citations for Human CD63 Antibody

Customer Reviews for Human CD63 Antibody (1)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23072732
    Species: Human
    Verified Customer | Posted 02/25/2015

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Protocols

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