Human CD63 Antibody

Catalog # Availability Size / Price Qty
MAB5048
MAB5048-SP
Detection of CD63 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (5)
FAQs
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Human CD63 Antibody Summary

Species Reactivity
Human
Specificity
Stains human CD63 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 460305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human CD63
Met1-Met238
Accession # P08962
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human PBMCs fixed with paraformaldehyde and permeabilized with saponin; Human PBMC monocytes (surface stain)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CD63 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD63 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were stained with Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunocytochemistry CD63 antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

CD63 in A549 Human Cell Line. CD63 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow (Cell Surface) View Larger

Detection of CD63 in PBMC monocytes by Flow Cytometry PBMC monocytes were stained with Mouse Anti-Human CD63 Monoclonal Antibody (Catalog # MAB5048, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

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Citations for Human CD63 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins
    Authors: Dmitry Ter-Ovanesyan, Tal Gilboa, Bogdan Budnik, Adele Nikitina, Sara Whiteman, Roey Lazarovits et al.
    eLife
  2. An immunogold single extracellular vesicular RNA and protein (Au SERP) biochip to predict responses to immunotherapy in non-small cell lung cancer patients
    Authors: Luong T. H. Nguyen, Jingjing Zhang, Xilal Y. Rima, Xinyu Wang, Kwang Joo Kwak, Tamio Okimoto et al.
    Journal of Extracellular Vesicles
  3. Framework for rapid comparison of extracellular vesicle isolation methods
    Authors: Dmitry Ter-Ovanesyan, Maia Norman, Roey Lazarovits, Wendy Trieu, Ju-Hyun Lee, George M Church et al.
    eLife
  4. Comparison and optimization of nanoscale extracellular vesicle imaging by scanning electron microscopy for accurate size-based profiling and morphological analysis†
    Authors: Sara Cavallaro, Petra Hååg, Kristina Viktorsson, Anatol Krozer, Kristina Fogel, Rolf Lewensohn et al.
    Nanoscale Advances
  5. Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles
    Authors: MP Zaborowski, K Lee, YJ Na, A Sammarco, X Zhang, M Iwanicki, PS Cheah, HY Lin, M Zinter, CY Chou, G Fulci, BA Tannous, CP Lai, MJ Birrer, R Weissleder, H Lee, XO Breakefiel
    Cell Rep, 2019-04-02;27(1):255-268.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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