Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 460305
View all CD63 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human CD63
Met1-Met238
Accession # P08962

Specificity

Stains human CD63 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD63 PE‑conjugated Antibody

Detection of CD63 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD63 in Human PBMC monocytes by Flow Cytometry.

PBMC monocytes were stained with Mouse Anti-Human CD63 PE‑conjugated Monoclonal Antibody (Catalog # IC5048P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD63 antibody in NS0 Mouse Cell Line Transfected with Human CD63 and eGFP antibody by Flow Cytometry.

Detection of CD63 in NS0 Mouse Cell Line Transfected with Human CD63 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with Human CD63 and eGFP was stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P). Quadrant markers were set based on control antibody staining (MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CD63 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: PBMC monocytes

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: NS0 mouse myeloma cell line transfected with Human CD63 and eGFP were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Entrez Gene IDs

967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)

Gene Symbol

CD63

UniProt

P08962

Additional CD63 Products

Product Documents for Human CD63 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD63 PE‑conjugated Antibody

For research use only

Citations for Human CD63 PE‑conjugated Antibody

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