Human CD63 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5048P-025
Detection of CD63 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (1)
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Human CD63 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CD63 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 460305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human CD63
Met1-Met238
Accession # P08962
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of CD63 in Human PBMCs by Flow Cytometry. View Larger

Detection of CD63 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of CD63 in NS0 Mouse Cell Line Transfected with Human CD63 and eGFP by Flow Cytometry. View Larger

Detection of CD63 in NS0 Mouse Cell Line Transfected with Human CD63 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with Human CD63 and eGFP was stained with Mouse Anti-Human CD63 PE-conjugated Monoclonal Antibody (Catalog # IC5048P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

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Citation for Human CD63 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance
    Authors: S Ma, LS Mangala, W Hu, E Bayaktar, A Yokoi, W Hu, S Pradeep, S Lee, PD Piehowski, A Villar-Pra, SY Wu, MH McGuire, OD Lara, C Rodriguez-, CJ LaFargue, NB Jennings, KD Rodland, T Liu, V Kundra, PT Ram, S Ramakrishn, G Lopez-Bere, RL Coleman, AK Sood
    Cell Reports, 2021;36(7):109549.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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