Human CD72 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF5405AFP680

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Sheep IgG
View all CD72 Primary Antibodies »

Product Specifications

Specificity

Detects human CD72 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human CD72 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD72

CD72, also known as Lyb‑2, is a 40‑45 kDa type II transmembrane glycoprotein that plays a role in immune system regulation (1). Mature human CD72 consists of a 95 amino acid (aa) cytoplasmic domain with two immunoreceptor tyrosine‑based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain with a coiled‑coil domain and a C‑type lectin domain (2). Within the ECD, human CD72 shares 48% and 44% aa sequence identity with mouse and rat CD72, respectively. CD72 is expressed on B lineage cells, NK cells, monocytes, dendritic cells, and mast cells (2‑6). CD72 binds to CD5 with mouse/human cross‑reactivity and to Semaphorin 4D/CD100 (5, 7‑9). It associates with CD79A in the B cell antigen receptor (BCR) complex following antigen stimulation and dampens BCR signaling through interactions with the phosphatase SHP‑1 (10). CD72 ligation with antibodies or with Semaphorin 4D induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP‑1, leading to B cell proliferation (5, 9). Both CD72 and Semaphorin 4D are required for the maintenance of B cell anergy and the regulation of peripheral B cell tolerance as shown by the development of autoimmunity in mice that lack either molecule (10, 11). In addition to its negative regulation of BCR signaling, CD72 can induce positive signaling in B cells independent of the BCR (12). CD72 binding to Semaphorin 4D induces cytokine production by monocytes and dendritic cells, inhibits SCF R/c‑kit induced mast cell proliferation and activation, and inhibits the cytolytic activity of NK cells (4‑6). Semaphorin 4D is expressed on activated NK cells and contributes to the adhesive interaction between NK and CD72+ target cells leading to a more efficent killing and enhanced IFN-gamma secretion (13).

References

  1. Nykimbeng-Takwi, E. and S.P. Chapoval (2011) Immunol. Res. 50:10.
  2. Von Hoegen, I. et al. (1990) J. Immunol. 144:4870.
  3. Nakayama, E. et al. (1989) Proc. Natl. Acad. Sci. 86:1352.
  4. Alcon, V.L. et al. (2009) Eur. J. Immunol. 39:826.
  5. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
  6. Kataoka, T.R. et al. (2010) J. Immunol. 184:2468.
  7. Van de Velde, H. et al. (1991) Nature 351:662.
  8. Luo, W. et al. (1992) J. Immunol. 148:1630.
  9. Kumanogoh, A. et al. (2000) Immunity 13:621.
  10. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
  11. Li, D. H.-H. et al. (2008) Arthritis Rheum. 58:3192.
  12. Wu, H.-J. et al. (2001) J. Immunol. 167:1263.
  13. Mizrahi, S. et al. (2007) PLoS ONE 9:e818.

Alternate Names

CD72, Ly-19.2, Ly-32.2, Lyb-2

Entrez Gene IDs

971 (Human); 12517 (Mouse)

Gene Symbol

CD72

UniProt

P21854

Additional CD72 Products

Product Documents for Human CD72 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD72 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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