Human CD72 Antibody

Catalog # Availability Size / Price Qty
AF5405-100
AF5405-SP
Detection of CD72 in Human PBMCs by Flow Cytometry.
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Human CD72 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD72 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD72
Arg117-Asp359
Accession # P21854
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD72 (Catalog # 5405-CD)
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD72 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD72 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Sheep Anti-Human CD72 Affinity-Purified Polyclonal Antibody (Catalog # AF5405) or (B) Sheep IgG Isotype Control (Catalog # 5-001-A) followed by anti-Sheep IgG APC-conjugated Secondary Antibody (Catalog # F0127) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD72

CD72, also known as Lyb‑2, is a 40‑45 kDa type II transmembrane glycoprotein that plays a role in immune system regulation (1). Mature human CD72 consists of a 95 amino acid (aa) cytoplasmic domain with two immunoreceptor tyrosine‑based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain with a coiled‑coil domain and a C‑type lectin domain (2). Within the ECD, human CD72 shares 48% and 44% aa sequence identity with mouse and rat CD72, respectively. CD72 is expressed on B lineage cells, NK cells, monocytes, dendritic cells, and mast cells (2‑6). CD72 binds to CD5 with mouse/human cross‑reactivity and to Semaphorin 4D/CD100 (5, 7‑9). It associates with CD79A in the B cell antigen receptor (BCR) complex following antigen stimulation and dampens BCR signaling through interactions with the phosphatase SHP‑1 (10). CD72 ligation with antibodies or with Semaphorin 4D induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP‑1, leading to B cell proliferation (5, 9). Both CD72 and Semaphorin 4D are required for the maintenance of B cell anergy and the regulation of peripheral B cell tolerance as shown by the development of autoimmunity in mice that lack either molecule (10, 11). In addition to its negative regulation of BCR signaling, CD72 can induce positive signaling in B cells independent of the BCR (12). CD72 binding to Semaphorin 4D induces cytokine production by monocytes and dendritic cells, inhibits SCF R/c‑kit induced mast cell proliferation and activation, and inhibits the cytolytic activity of NK cells (4‑6). Semaphorin 4D is expressed on activated NK cells and contributes to the adhesive interaction between NK and CD72+ target cells leading to a more efficent killing and enhanced IFN-gamma secretion (13).

References
  1. Nykimbeng-Takwi, E. and S.P. Chapoval (2011) Immunol. Res. 50:10.
  2. Von Hoegen, I. et al. (1990) J. Immunol. 144:4870.
  3. Nakayama, E. et al. (1989) Proc. Natl. Acad. Sci. 86:1352.
  4. Alcon, V.L. et al. (2009) Eur. J. Immunol. 39:826.
  5. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
  6. Kataoka, T.R. et al. (2010) J. Immunol. 184:2468.
  7. Van de Velde, H. et al. (1991) Nature 351:662.
  8. Luo, W. et al. (1992) J. Immunol. 148:1630.
  9. Kumanogoh, A. et al. (2000) Immunity 13:621.
  10. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
  11. Li, D. H.-H. et al. (2008) Arthritis Rheum. 58:3192.
  12. Wu, H.-J. et al. (2001) J. Immunol. 167:1263.
  13. Mizrahi, S. et al. (2007) PLoS ONE 9:e818.
Entrez Gene IDs
971 (Human); 12517 (Mouse)
Alternate Names
CD72 antigenB-cell differentiation antigen CD72; CD72 molecule; CD72; CD72b; Ly-19.2; Ly-32.2; Lyb-2; LYB2lyb-2

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