Human CEACAM-1/CD66a Alexa Fluor® 647-conjugated Antibody

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FAB2244R-100UG
R&D Systems Antibodies
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Human CEACAM-1/CD66a Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1.
Source
Monoclonal Mouse IgG2b Clone # 283340
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM-1
Gln35-Gly428
Accession # P13688
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood granulocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CEACAM-1/CD66a

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3, and 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6-8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145-317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V‑type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear. Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V‑type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Thompson, J. et al. (1992) Genomics 12:761.
  3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
  4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
  5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
  6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
  7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
  8. Watt, S.M. et al. (1994) Blood 84:200.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
  11. Bogoevska, V. et al. (2005) Glycobiology 16:197.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Entrez Gene IDs
634 (Human); 26365 (Mouse); 102139857 (Cynomolgus Monkey)
Alternate Names
antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR

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