Human CEACAM-5/CD66e Alexa Fluor® 647-conjugated Antibody Summary
Accession # ABM87752
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-5/CD66e or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM‑5/CD66e Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB41281R). Quadrant markers were set based on control antibody staining (IC003R). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM‑5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6‑8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK‑mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14‑16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).
- Zebhauser, R. et al. (2005) Genomics 86:566.
- Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
- Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
- Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
- Garcia, M. et al. (1991) Cancer Res. 51:5679.
- Stern, N. et al. (2005) J. Immunol. 174:6692.
- Benchimol, S. et al. (1989) Cell 57:327.
- Zhou, H. et al. (1993) J. Cell Biol. 122:951.
- Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
- Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
- Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
- Ordonez, C. et al. (2000) Cancer Res. 60:3419.
- Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
- Chen, T. et al. (1997) J. Exp. Med. 185:1557.
- Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
- Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human CEACAM-5/CD66e Alexa Fluor® 647-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human CEACAM-5/CD66e Alexa Fluor® 647-conjugated Antibody and earn rewards!
Have you used Human CEACAM-5/CD66e Alexa Fluor® 647-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image