Human CEACAM-5/CD66e APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB41281A
Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human CEACAM-5/CD66e APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM‑5/CD66e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1 or -6 is observed.
Source
Monoclonal Mouse IgG2A Clone # 487609
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM‑5/CD66e
Lys35-Ala685
Accession # ABM87752
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CEACAM-5/CD66e antibody in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP antibody by Flow Cytometry. View Larger

Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-5/CD66e or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM-5/CD66e APC-conjugated Monoclonal Antibody (Catalog # FAB41281A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM-5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6-8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14-16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References
  1. Zebhauser, R. et al. (2005) Genomics 86:566.
  2. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
  3. Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
  4. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
  5. Garcia, M. et al. (1991) Cancer Res. 51:5679.
  6. Stern, N. et al. (2005) J. Immunol. 174:6692.
  7. Benchimol, S. et al. (1989) Cell 57:327.
  8. Zhou, H. et al. (1993) J. Cell Biol. 122:951.
  9. Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
  10. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
  11. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
  12. Ordonez, C. et al. (2000) Cancer Res. 60:3419.
  13. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
  14. Chen, T. et al. (1997) J. Exp. Med. 185:1557.
  15. Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
  16. Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Entrez Gene IDs
1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)
Alternate Names
Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100

Product Datasheets

You must select a language.

x

Citation for Human CEACAM-5/CD66e APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Near-Infrared Fluorescence Imaging of Pancreatic Cancer Using a Fluorescently Labelled Anti-CEA Nanobody Probe: A Preclinical Study
    Authors: Labrinus van Manen, Lizzie D. A. N. de Muynck, Victor M. Baart, Shadhvi Bhairosingh, Pieterjan Debie, Alexander L. Vahrmeijer et al.
    Biomolecules

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CEACAM-5/CD66e APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human CEACAM-5/CD66e APC-conjugated Antibody and earn rewards!

Have you used Human CEACAM-5/CD66e APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review