Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, ELISA Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 487609
View all CEACAM-5/CD66e Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM‑5/CD66e
Lys35-Ala685
Accession # ABM87752

Specificity

Detects human CEACAM‑5/CD66e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1 or -6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CEACAM‑5/CD66e Antibody

Detection of CEACAM-5/CD66e antibody in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP antibody by Flow Cytometry.

Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-5/CD66e or (B) human CEACAM-8/CD66b and eGFP was stained with Mouse Anti-Human CEACAM-5/CD66e Monoclonal Antibody (Catalog # MAB41281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
CEACAM-5/CD66e antibody in Human Colon by Immunohistochemistry (IHC-P).

CEACAM‑5/CD66e in Human Colon.

CEACAM-5/CD66e was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CEACAM-5/CD66e Monoclonal Antibody (Catalog # MAB41281) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CEACAM‑5/CD66e Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human CEACAM-5/CD66e and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: Recombinant Human CEACAM‑5/CD66e (Catalog # 4128-CM)

Reviewed Applications

Read 5 reviews rated 4.8 using MAB41281 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM‑5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6‑8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK‑mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14‑16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

  1. Zebhauser, R. et al. (2005) Genomics 86:566.
  2. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
  3. Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
  4. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
  5. Garcia, M. et al. (1991) Cancer Res. 51:5679.
  6. Stern, N. et al. (2005) J. Immunol. 174:6692.
  7. Benchimol, S. et al. (1989) Cell 57:327.
  8. Zhou, H. et al. (1993) J. Cell Biol. 122:951.
  9. Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
  10. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
  11. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
  12. Ordonez, C. et al. (2000) Cancer Res. 60:3419.
  13. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
  14. Chen, T. et al. (1997) J. Exp. Med. 185:1557.
  15. Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
  16. Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Gene Symbol

CEACAM5

UniProt

ABM87752

Additional CEACAM-5/CD66e Products

Product Documents for Human CEACAM‑5/CD66e Antibody

Certificate of Analysis

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Product Specific Notices for Human CEACAM‑5/CD66e Antibody

For research use only

Citations for Human CEACAM‑5/CD66e Antibody

Customer Reviews for Human CEACAM‑5/CD66e Antibody (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Human CEACAM-5/CD66e Antibody
    Name: Lucas Baumard
    Application: Flow Cytometry
    Sample Tested: Colon cancer cell line
    Species: Human
    Verified Customer | Posted 07/29/2025
    Unstained vs stained in mid-CEA-expressing cell line
    Human CEACAM‑5/CD66e Antibody MAB41281
  • Human CEACAM-5/CD66e Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 10/02/2021
    Human CEACAM‑5/CD66e Antibody MAB41281
  • Human CEACAM-5/CD66e Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tumor cell line
    Species: Human
    Verified Customer | Posted 08/16/2021
    Human CEACAM‑5/CD66e Antibody MAB41281
  • Human CEACAM-5/CD66e Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: tumor cell lines
    Species: Human
    Verified Customer | Posted 03/23/2018
  • Human CEACAM-5/CD66e Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 10/31/2017

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