Human CEACAM-5/CD66e Antibody

Catalog # Availability Size / Price Qty
MAB41281
MAB41281-SP
Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (4)

Human CEACAM-5/CD66e Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM‑5/CD66e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1 or -6 is observed.
Source
Monoclonal Mouse IgG2A Clone # 487609
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM‑5/CD66e
Lys35-Ala685
Accession # ABM87752
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CEACAM‑5/CD66e (Catalog # 4128-CM)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CEACAM-5/CD66e antibody in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP antibody by Flow Cytometry. View Larger

Detection of CEACAM‑5/CD66e in HEK293 Human Cell Line Transfected with Human CEACAM-5/CD66e and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-5/CD66e or (B) human CEACAM-8/CD66b and eGFP was stained with Mouse Anti-Human CEACAM-5/CD66e Monoclonal Antibody (Catalog # MAB41281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Immunohistochemistry CEACAM-5/CD66e antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

CEACAM‑5/CD66e in Human Colon. CEACAM-5/CD66e was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CEACAM-5/CD66e Monoclonal Antibody (Catalog # MAB41281) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA and CD66e, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM molecules are either transmembrane or GPI-linked, and are differentially expressed between species (1, 2). Orthologs of human CEACAM‑5 have not been described in other species. CEACAM-5, which is expressed primarily by epithelial cells, consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2‑4). CEACAM-5 is synthesized as a 180 kDa, variably glycosylated molecule of which approximately 60% is carbohydrate (5). CEACAM-5 functions as a calcium‑independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (6‑8). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (7). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is upregulated in a wide variety of human tumors and is a commonly used cancer marker (9). It promotes tumor cell migration, invasion, adhesion, and metastasis (10). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (11, 12). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (13). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK‑mediated lysis (6). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14‑16). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References
  1. Zebhauser, R. et al. (2005) Genomics 86:566.
  2. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
  3. Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738.
  4. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
  5. Garcia, M. et al. (1991) Cancer Res. 51:5679.
  6. Stern, N. et al. (2005) J. Immunol. 174:6692.
  7. Benchimol, S. et al. (1989) Cell 57:327.
  8. Zhou, H. et al. (1993) J. Cell Biol. 122:951.
  9. Goldenberg, D.M. et al. (1976) J. Natl. Cancer Inst. 57:11.
  10. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
  11. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
  12. Ordonez, C. et al. (2000) Cancer Res. 60:3419.
  13. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
  14. Chen, T. et al. (1997) J. Exp. Med. 185:1557.
  15. Bos, M.P. et al. (1997) Infect. Immun. 65:2353.
  16. Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Entrez Gene IDs
1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)
Alternate Names
Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100

Product Datasheets

You must select a language.

x

Citations for Human CEACAM-5/CD66e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Multiomic analysis reveals cellular and epigenetic plasticity in intestinal pouches of ulcerative colitis patients
    Authors: Zhao, Y;Zhou, R;Xie, B;Liu, CY;Kalski, M;Cham, CM;Koval, J;Weber, CR;Rubin, DT;Sogin, M;Crosson, S;Huang, J;Fiebig, A;Dalal, S;Chang, EB;Basu, A;Pott, S;
    medRxiv : the preprint server for health sciences
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. A phase 2 study of GVAX colon vaccine with cyclophosphamide and pembrolizumab in patients with mismatch repair proficient advanced colorectal cancer
    Authors: M Yarchoan, CY Huang, Q Zhu, AK Ferguson, JN Durham, RA Anders, ED Thompson, NS Rozich, DL Thomas, JM Nauroth, C Rodriguez, A Osipov, A De Jesus-A, DT Le, AG Murphy, D Laheru, RC Donehower, EM Jaffee, L Zheng, NS Azad
    Cancer Med, 2019-12-26;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Control
  3. Potentials and capabilities of the Extracellular Vesicle (EV) Array.
    Authors: Jorgensen MM, Baek R, Varming K.
    J Extracell Vesicles

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CEACAM-5/CD66e Antibody

Average Rating: 4.8 (Based on 4 Reviews)

5 Star
75%
4 Star
25%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human CEACAM-5/CD66e Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human CEACAM-5/CD66e Antibody
By Anonymous on 10/02/2021
Application: IHC Sample Tested: Colon tissue Species: Human

Human CEACAM-5/CD66e Antibody
By Anonymous on 08/16/2021
Application: WB Sample Tested: Tumor cell line Species: Human

Human CEACAM-5/CD66e Antibody
By Anonymous on 03/23/2018
Application: ELISA Sample Tested: tumor cell lines Species: Human

Human CEACAM-5/CD66e Antibody
By Anonymous on 10/31/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Human