Detection of ChemR23 in Human Whole Blood Monocytes by Flow Cytometry.
Human whole blood monocytes were stained with Mouse Anti-Human IL-3 R alpha/CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human ChemR23 APC‑conjugated Monoclonal Antibody (Catalog # FAB362A) or (B) Mouse IgG3 Allophycocyanin Isotype Control (Catalog # IC007A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
ChemR23, also known as CMKLR1, is a 47-48 kDa 7-transmembrane member of the rhodopsin, or G-protein coupled receptor family 1. It has restricted expression on select cell types that, in primate, include adipocytes, astrocytes, endothelial cells, microglia/macrophages, chondrocytes, monocytes, osteocytes, NK cells, and plasmacytoid plus myeloid dendritic cells. In mouse, would studies show ChemR23 to be expressed by neutrophils and keratinocytes. The universally-agreed on ligand for ChemR23 is Chemerin, a 137 amino acid (aa) non-chemokine product of fibroblasts, platelets, adipocytes and endothelium. The Chermerin:ChemR23 system is suggested to promote chemotaxis for all ChemR23+ cells, and to induce angiogenesis via endothelial cell migration and MMP-2 and -9 activation. There is one alternative splice form that shows a deletion of aa 1 and 2 (NP_004063). Over the extracellular domains of ChemR23, human and mouse share 69% aa sequence identity.
Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23
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