Human Chitinase 3-like 2 Biotinylated Antibody

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BAF5116
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Human Chitinase 3-like 2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chitinase 3-like 2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human CHIT-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 2
Tyr27-Leu390
Accession # Q15782
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Chitinase 3-like 2 (Catalog # 5112-CH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 2

Chitinase 3-like 2 (CHI3L2), also called chondrocyte protein 39 or YKL-39, is a 39 kDa potentially glycosylated member of the glycosyl hydrolase 18 family (1‑3). It is secreted by synovial fibroblasts, chondrocytes, and TGF beta /IL-4-stimulated monocyte-derived macrophages (1, 4, 5). The human CHI3L2 cDNA encodes 390 amino acids (aa), including a 26 aa signal sequence and a 364 aa mature region. Potential alternate start sites at Met-24 and Met+80 would create isoforms of 413 aa and 311 aa, while a potential deletion of aa 15‑24 would create a 380 aa isoform (6). Mature human CHI3L2 shares 89%, 87% and 84% aa sequence identity with bovine, porcine and equine CHI3L2, respectively. The rodent genome does not include a CHI3L2 ortholog (2). Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1‑3). Unlike CHI3L1, CHI3L2 does not bind heparin (1). While CHI3L1 is the major protein excreted by chondrocytes, up-regulation of CHI3L2 and not CHI3L1 correlates with osteoarthritic cartilage degeneration (1, 7, 8). CHI3L2 has been proposed as a potential biomarker for osteoarthritis (4, 7, 8). CHI3L1 has been found to coordinate adhesion receptors and promote cell signaling and tumor angiogenesis; like CHI3L1, CHI3L2 can enhance cell adhesion (9).

Entrez Gene IDs
1117 (Human)
Alternate Names
CHI3L2; Chitinase 3 like 2; Chitinase 3-like 2; chitinase-3-like protein 2; Chondrocyte protein 39; YKL39; YKL-39YKL39

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