Human Choline Acetyltransferase/ChAT Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB3447R Datasheet / COA / SDS

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FAB3447R-100UG

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All Choline Acetyltransferase/ChAT Antibodies

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Human Choline Acetyltransferase/ChAT Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Choline Acetyltransferase/ChAT in direct ELISAs and Western blots. Recognizes both ChAT isoform 1 (ChAT-69) and isoform 2 (ChAT-82).

Source

Monoclonal Mouse IgG1 Clone # 334008

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Choline Acetyltransferase/ChAT isoform 1
Ala2-Pro630
Accession # NP_066266

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Choline Acetyltransferase/ChAT

ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinogenic neuron communication. It serves as a marker for functional cholinogenic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N‑terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.

Entrez Gene IDs

1103 (Human); 12647 (Mouse); 290567 (Rat)

Alternate Names

acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; Choline Acetyltransferase; choline O-acetyltransferase; CMS1A; CMS1A2; EC 2.3.1; EC 2.3.1.6