Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.
Human Coagulation Factor III/Tissue Factor Quantikine ELISA
R&D Systems | Catalog # DCF300
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL), Urine (50 µL)
Sensitivity
2.05 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
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Product Summary for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulation Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Coagulation Factor III
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 33.4 | 65.4 | 148 | 71 | 144 | 301 |
| Standard Deviation | 0.78 | 1.81 | 5.0 | 4.5 | 7.8 | 17.1 |
| CV% | 2.3 | 2.8 | 3.4 | 6.3 | 5.4 | 5.7 |
Recovery for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
The recovery of Coagulation Factor III spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 95-110 |
| Citrate Plasma (n=4) | 92 | 86-102 |
| EDTA Plasma (n=4) | 91 | 86-97 |
| Heparin Plasma (n=4) | 95 | 88-109 |
| Urine (n=4) | 102 | 96-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Coagulation Factor III were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
Human Coagulation Factor III/Tissue Factor ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Coagulation Factor III/Tissue Factor
Alternate Names
CD142, F3, Thromboplastin, Tissue Factor
Gene Symbol
F3
Additional Coagulation Factor III/Tissue Factor Products
Product Documents for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
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Customer Reviews for Human Coagulation Factor III/Tissue Factor Quantikine ELISA (2)
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2 Customer Ratings
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Sample Tested: Serum and Plasma and Lung cancer tissueVerified Customer | Posted 10/31/2023The concentration titration uses a log2 algorithm, and we employed an intermittent dilution method. Although the results were not as expected, the detection indicator is still sensitive.
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Sample Tested: Cell Culture MediaVerified Customer | Posted 05/12/2017Selected cell lines were split into various flasks, centrifuged and supernatant used for ELISA
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Protocols
View specific protocols for Human Coagulation Factor III/Tissue Factor Quantikine ELISA (DCF300):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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