Human COMP Quantikine ELISA Kit

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DCMP0
Control Products Available
Human COMP/Thrombospondin-5 ELISA Standard Curve
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Product Details
Procedure
Citations (11)
FAQs
Supplemental Products
Reviews (2)

Human COMP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sensitivity
0.036 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human COMP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human COMP immunoassay is a 4.5 hour solid phase ELISA designed to measure human COMP in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human COMP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human COMP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring COMP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 0.9 2.92 6.11 1.05 3.07 6.26
Standard Deviation 0.028 0.112 0.225 0.045 0.146 0.3
CV% 2.1 3.8 3.7 4.3 4.8 4.8

Recovery

The recovery of COMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 103 95-111
Cell Lysates (n=1) 102 98-108
EDTA Plasma (n=4) 103 86-114
Heparin Plasma (n=4) 99 88-112
Serum (n=4) 102 86-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human COMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human COMP/Thrombospondin-5 ELISA Linearity

Scientific Data

Human COMP/Thrombospondin-5 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: COMP/Thrombospondin-5

COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.

Long Name:
Cartilage Oligomeric Matrix Protein
Entrez Gene IDs:
1311 (Human); 12845 (Mouse)
Alternate Names:
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human COMP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Associations between serum biomarkers of cartilage metabolism and serum hyaluronic acid, with risk factors, pain categories, and disease severity in knee osteoarthritis: a pilot study
    Authors: C Papaneophy, A Alabajos-C, E Viosca-Her, C Calvis, M Costa, AE Christodou, A Kroushovsk, A Lapithis, VM Lapithi, I Papayianni, A Christou, R Messeguer, C Giannaki, K Felekkis
    Bmc Musculoskeletal Disorders, 2022;23(1):195.
    Species: Human
    Sample Types: Serum
  2. Comparison of Extracorporeal Shockwave Therapy with Non-Steroid Anti-Inflammatory Drugs and Intra-Articular Hyaluronic Acid Injection for Early Osteoarthritis of the Knees
    Authors: SW Jhan, CJ Wang, KT Wu, KK Siu, JY Ko, WC Huang, WY Chou, JH Cheng
    Biomedicines, 2022;10(2):.
    Species: Human
    Sample Types: Serum
  3. The Effect of 13 Weeks Long-Distance Bicycle Riding on Inflammatory Response Indicators Related to Joint Cartilage and Muscle Damage
    Authors: HJ Kim, HC Lee
    International Journal of Environmental Research and Public Health, 2022;19(23):.
    Species: Human
    Sample Types: Plasma
  4. Cartilage oligomeric matrix protein is an endogenous &beta-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury
    Authors: Y Fu, Y Huang, Z Yang, Y Chen, J Zheng, C Mao, Z Li, Z Liu, B Yu, T Li, M Wang, C Xu, Y Zhou, G Zhao, Y Jia, W Guo, X Jia, T Zhang, L Li, Z Liu, S Guo, M Ma, H Zhang, B Liu, J Du, W Wang, C Tang, P Gao, Q Xu, X Wang, J Liu, J Sun, W Kong
    Cell Research, 2021;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Association of Circulating COMP and YKL-40 as Markers of Metabolic Changes of Cartilage with Adipocytokines in Juvenile Idiopathic Arthritis
    Authors: K Winsz-Szcz, K Ku?nik-Tro, A Gruenpeter, M Wojdas, K D?bkowska, K Olczyk
    Metabolites, 2020;10(2):.
    Species: Human
    Sample Types: Serum
  6. Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12
    Authors: S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP
    Authors: L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu
    J. Exp. Clin. Cancer Res., 2019;38(1):170.
    Species: Human
    Sample Types: Cell Culture S
  8. Increasing Incidence of Colon Cancer in the Young: Assessing the Tumor Biology
    Authors: VN Nfonsam, H Jecius, D Chen, PN Omesiete, AN Ewongwo, E Elquza, AJ Scott, J Jandova
    J. Am. Coll. Surg., 2019;0(0):.
    Species: Human
    Sample Types: Serum
  9. The Effects of Well-Rounded Exercise Program on Systemic Biomarkers Related to Cartilage Metabolism
    Authors: M Azukizawa, H Ito, Y Hamamoto, T Fujii, Y Morita, A Okahata, T Tomizawa, M Furu, K Nishitani, S Kuriyama, S Nakamura, H Yoshitomi, T Nakatani, T Tsuboyama, M Hamaguchi, S Matsuda, T Yasuda
    Cartilage, 2018;0(0):1947603518767.
    Species: Human
    Sample Types: Serum
  10. Effects of dietary supplementation with a standardized aqueous extract of Terminalia chebula fruit (AyuFlex(�)) on joint mobility, comfort, and functional capacity in healthy overweight subjects: a randomized placebo-controlled clinical trial
    Authors: HL Lopez, SM Habowski, JE Sandrock, B Raub, A Kedia, EJ Bruno, TN Ziegenfuss
    BMC Complement Altern Med, 2017;17(1):475.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human COMP Quantikine ELISA Kit

Average Rating: 4.5 (Based on 2 Reviews)

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Human COMP Quantikine ELISA Kit
By Anonymous on 10/04/2020
Sample Tested: Serum

Human COMP Quantikine ELISA Kit
By Jessica Fernando on 10/23/2017
Sample Tested: Serum